Three-dimensional label-free observation of individual bacteria upon antibiotic treatment using optical diffraction tomography

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Measuring alterations in bacteria upon antibiotic application is important for basic studies in microbiology, drug discovery, clinical diagnosis, and disease treatment. However, imaging and 3D time-lapse response analysis of individual bacteria upon antibiotic application remain largely unexplored mainly due to limitations in imaging techniques. Here, we present a method to systematically investigate the alterations in individual bacteria in 3D and quantitatively analyze the effects of antibiotics. Using optical diffraction tomography, in-situ responses of Escherichia coli and Bacillus subtilis to various concentrations of ampicillin were investigated in a label-free and quantitative manner. The presented method reconstructs the dynamic changes in the 3D refractive-index distributions of living bacteria in response to antibiotics at sub-micrometer spatial resolution.
Publisher
OPTICAL SOC AMER
Issue Date
2020-02
Language
English
Article Type
Article
Citation

BIOMEDICAL OPTICS EXPRESS, v.11, no.3, pp.377740 - 1267

ISSN
2156-7085
DOI
10.1364/BOE.377740
URI
http://hdl.handle.net/10203/273750
Appears in Collection
PH-Journal Papers(저널논문)NT-Journal Papers(저널논문)
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