Enhanced production of cellulose in Komagataeibacter xylinus by preventing insertion of IS element into cellulose synthesis gene

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dc.contributor.authorHur, Dong Hoonko
dc.contributor.authorRhee, Hong-Soonko
dc.contributor.authorLee, Jae Hyungko
dc.contributor.authorShim, Woo Yongko
dc.contributor.authorKim, Tae Yongko
dc.contributor.authorLee, Sang Yupko
dc.contributor.authorPark, Jin Hwanko
dc.contributor.authorJeong, Ki Junko
dc.date.accessioned2020-03-23T08:20:10Z-
dc.date.available2020-03-23T08:20:10Z-
dc.date.created2020-03-18-
dc.date.created2020-03-18-
dc.date.issued2020-04-
dc.identifier.citationBIOCHEMICAL ENGINEERING JOURNAL, v.156, pp.107527-
dc.identifier.issn1369-703X-
dc.identifier.urihttp://hdl.handle.net/10203/273385-
dc.description.abstractBacterial cellulose consisting of pure cellulose nanofibers, is a promising biomaterial with versatile applications. The primary producer is Komagataeibacter xylinus, however, its use is limited because the bacteria undergo irreversible conversion into a non-cellulose-producing mutant (Cel(-)) during shaking cultivation. Here, by investigating the bcs operon in Cel(-) mutants, we found that an IS element-inserted mutation in bcsA, is responsible for the conversion into Cel(-). To prevent IS element insertion into bcsA, putative IS element recognition sequences were modified. As a result, the engineered strain retained its own bcsA gene over seven sub-cultivations, and showed 1.7-fold higher productivity than wild-type strains without any differences of physical and chemical properties.-
dc.languageEnglish-
dc.publisherELSEVIER-
dc.titleEnhanced production of cellulose in Komagataeibacter xylinus by preventing insertion of IS element into cellulose synthesis gene-
dc.typeArticle-
dc.identifier.wosid000518151700020-
dc.identifier.scopusid2-s2.0-85079002667-
dc.type.rimsART-
dc.citation.volume156-
dc.citation.beginningpage107527-
dc.citation.publicationnameBIOCHEMICAL ENGINEERING JOURNAL-
dc.identifier.doi10.1016/j.bej.2020.107527-
dc.contributor.localauthorLee, Sang Yup-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.nonIdAuthorRhee, Hong-Soon-
dc.contributor.nonIdAuthorShim, Woo Yong-
dc.contributor.nonIdAuthorKim, Tae Yong-
dc.contributor.nonIdAuthorPark, Jin Hwan-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorBacterial cellulose-
dc.subject.keywordAuthorKomagataeibacterxylinus-
dc.subject.keywordAuthorIS element-
dc.subject.keywordAuthorbcsA-
dc.subject.keywordPlusBACTERIAL-CELLULOSE-
dc.subject.keywordPlusGLUCONACETOBACTER-XYLINUS-
dc.subject.keywordPlusBIOSYNTHESIS-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusHANSENII-
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