DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hur, Dong Hoon | ko |
dc.contributor.author | Rhee, Hong-Soon | ko |
dc.contributor.author | Lee, Jae Hyung | ko |
dc.contributor.author | Shim, Woo Yong | ko |
dc.contributor.author | Kim, Tae Yong | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Park, Jin Hwan | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2020-03-23T08:20:10Z | - |
dc.date.available | 2020-03-23T08:20:10Z | - |
dc.date.created | 2020-03-18 | - |
dc.date.created | 2020-03-18 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | BIOCHEMICAL ENGINEERING JOURNAL, v.156, pp.107527 | - |
dc.identifier.issn | 1369-703X | - |
dc.identifier.uri | http://hdl.handle.net/10203/273385 | - |
dc.description.abstract | Bacterial cellulose consisting of pure cellulose nanofibers, is a promising biomaterial with versatile applications. The primary producer is Komagataeibacter xylinus, however, its use is limited because the bacteria undergo irreversible conversion into a non-cellulose-producing mutant (Cel(-)) during shaking cultivation. Here, by investigating the bcs operon in Cel(-) mutants, we found that an IS element-inserted mutation in bcsA, is responsible for the conversion into Cel(-). To prevent IS element insertion into bcsA, putative IS element recognition sequences were modified. As a result, the engineered strain retained its own bcsA gene over seven sub-cultivations, and showed 1.7-fold higher productivity than wild-type strains without any differences of physical and chemical properties. | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Enhanced production of cellulose in Komagataeibacter xylinus by preventing insertion of IS element into cellulose synthesis gene | - |
dc.type | Article | - |
dc.identifier.wosid | 000518151700020 | - |
dc.identifier.scopusid | 2-s2.0-85079002667 | - |
dc.type.rims | ART | - |
dc.citation.volume | 156 | - |
dc.citation.beginningpage | 107527 | - |
dc.citation.publicationname | BIOCHEMICAL ENGINEERING JOURNAL | - |
dc.identifier.doi | 10.1016/j.bej.2020.107527 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Rhee, Hong-Soon | - |
dc.contributor.nonIdAuthor | Shim, Woo Yong | - |
dc.contributor.nonIdAuthor | Kim, Tae Yong | - |
dc.contributor.nonIdAuthor | Park, Jin Hwan | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bacterial cellulose | - |
dc.subject.keywordAuthor | Komagataeibacterxylinus | - |
dc.subject.keywordAuthor | IS element | - |
dc.subject.keywordAuthor | bcsA | - |
dc.subject.keywordPlus | BACTERIAL-CELLULOSE | - |
dc.subject.keywordPlus | GLUCONACETOBACTER-XYLINUS | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | HANSENII | - |
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