Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

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dc.contributor.authorKim, Hee Suko
dc.contributor.authorPark, Won-Kunko
dc.contributor.authorLee, Bongsooko
dc.contributor.authorSeon, Gyeonghoko
dc.contributor.authorSuh, William, Iko
dc.contributor.authorMoon, Myounghoonko
dc.contributor.authorChang, Yong Keunko
dc.date.accessioned2020-02-05T09:20:40Z-
dc.date.available2020-02-05T09:20:40Z-
dc.date.created2020-02-04-
dc.date.created2020-02-04-
dc.date.created2020-02-04-
dc.date.issued2019-12-
dc.identifier.citationSCIENTIFIC REPORTS, v.9, pp.19383-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10203/272131-
dc.description.abstractThe heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a three-fold improvement in biomass amounts, from 5.85 to 18.13 g/L, in comparison to a non-optimized BG11 medium containing 72 g/L glucose. Scaling up the cultivation to a 5-L fermenter resulted in a greatly improved biomass concentration of 35.3 g/L owing to more efficient oxygenation of the culture. In addition, phosphorus feeding fermentation was employed in an effort to address early depletion of phosphate, and a maximum biomass concentration of 42.95 g/L was achieved, with biomass productivity of 5.37 g/L/D.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleOptimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation-
dc.typeArticle-
dc.identifier.wosid000507593000001-
dc.identifier.scopusid2-s2.0-85076889133-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.beginningpage19383-
dc.citation.publicationnameSCIENTIFIC REPORTS-
dc.identifier.doi10.1038/s41598-019-55854-9-
dc.contributor.localauthorChang, Yong Keun-
dc.contributor.nonIdAuthorPark, Won-Kun-
dc.contributor.nonIdAuthorLee, Bongsoo-
dc.contributor.nonIdAuthorSuh, William, I-
dc.contributor.nonIdAuthorMoon, Myounghoon-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusLIPID RICH CULTIVATION-
dc.subject.keywordPlusMICROALGAE PRODUCTION-
dc.subject.keywordPlusLOW-COST-
dc.subject.keywordPlusSOROKINIANA-
dc.subject.keywordPlusBIODIESEL-
dc.subject.keywordPlusBIOMASS-
dc.subject.keywordPlusWASTE-
dc.subject.keywordPlusPROTOTHECOIDES-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusGLUCOSE-
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CBE-Journal Papers(저널논문)
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