PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood

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dc.contributor.authorLee, Dong Yunko
dc.contributor.authorKang, Sukmoko
dc.contributor.authorLee, Yonghyunko
dc.contributor.authorKim, Jin Yongko
dc.contributor.authorYoo, Dohyunko
dc.contributor.authorJung, Wonsikko
dc.contributor.authorLee, Soyoungko
dc.contributor.authorJeong, Yong Yeonko
dc.contributor.authorLee, Kwangyeolko
dc.contributor.authorJon, Sangyongko
dc.date.accessioned2020-02-05T09:20:32Z-
dc.date.available2020-02-05T09:20:32Z-
dc.date.created2020-02-04-
dc.date.created2020-02-04-
dc.date.issued2020-02-
dc.identifier.citationTHERANOSTICS, v.10, no.5, pp.1997 - 2007-
dc.identifier.issn1838-7640-
dc.identifier.urihttp://hdl.handle.net/10203/272130-
dc.description.abstractRationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been documented. Methods: To this end, we constructed a biosensor for ROS detection based on PEGylated bilirubin (PEG-BR)-coated SPIONs (PEG-BR@SPIONs) that were prepared by simple sonication via ligand exchange. In addition, near infra-red (NIR) fluorescent dye was loaded onto PEG-BR@SPIONs as a secondary option for fluorescence-based ROS detection. Results: PEG-BR@SPIONs showed high colloidal stability under physiological conditions, but upon exposure to the model ROS, NaOCl, in vitro, they aggregated, causing a decrease in signal intensity in T2-weighted MR images. Furthermore, ROS-responsive PEG-BR@SPIONs were taken up by lipopolysaccharide (LPS)-activated macrophages to a much greater extent than ROS-unresponsive control nanoparticles (PEG-DSPE@SPIONs). In a sepsis-mimetic clinical setting, PEG-BR@SPIONs were able to directly detect the concentrations of ROS in whole blood samples through a clear change in T2 MR signals and a 'turn-on' signal of fluorescence. Conclusions: These findings suggest that PEG-BR@SPIONs have the potential as a new type of dual mode (MRSw-based and fluorescence-based) biosensors for ROS detection and could be used to diagnose many diseases associated with ROS overproduction.-
dc.languageEnglish-
dc.publisherIVYSPRING INT PUBL-
dc.titlePEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood-
dc.typeArticle-
dc.identifier.wosid000508008300002-
dc.identifier.scopusid2-s2.0-85078221743-
dc.type.rimsART-
dc.citation.volume10-
dc.citation.issue5-
dc.citation.beginningpage1997-
dc.citation.endingpage2007-
dc.citation.publicationnameTHERANOSTICS-
dc.identifier.doi10.7150/thno.39662-
dc.contributor.localauthorJon, Sangyong-
dc.contributor.nonIdAuthorLee, Yonghyun-
dc.contributor.nonIdAuthorJeong, Yong Yeon-
dc.contributor.nonIdAuthorLee, Kwangyeol-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorBilirubin nanoparticles-
dc.subject.keywordAuthorBiosensors-
dc.subject.keywordAuthorIron oxide nanoparticles-
dc.subject.keywordAuthorMagnetic relaxation switching-
dc.subject.keywordAuthorReactive oxygen species-
dc.subject.keywordPlusREACTIVE OXYGEN-
dc.subject.keywordPlusPHOTOTHERMAL THERAPY-
dc.subject.keywordPlusHUMAN-NEUTROPHILS-
dc.subject.keywordPlusCHLORINATION-
dc.subject.keywordPlusPHAGOCYTOSIS-
dc.subject.keywordPlusINFLAMMATION-
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