Mechanism for coordinate regulation of rpoS by sRNA-sRNA interaction in Escherichia coli

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RpoS is a key regulator of general stress responses in Escherichia coli. Its expression is post-transcriptionally up-regulated by the small RNAs (sRNAs), ArcZ, DsrA and RprA, through sRNA-rpoS mRNA interactions. Although overexpression of the sRNA, CyaR, was reported to down-regulate rpoS expression, how CyaR regulates rpoS has not been determined. Here, we report that CyaR represses rpoS expression by base-pairing with a region next to the ArcZ binding site in the 5MODIFIER LETTER PRIME UTR of rpoS mRNA and that CyaR expression itself is down-regulated by ArcZ through sRNA-sRNA interaction. The short form of ArcZ, but not the full-length form, can base-pair with CyaR. This ArcZ-CyaR interaction triggers degradation of CyaR by RNase E, alleviating the CyaR-mediated rpoS repression. These results suggest that ArcZ not only participates in rpoS translation as an activator, but also acts as a regulator of the reciprocally acting CyaR, maximizing its rpoS-activating effect.
Publisher
TAYLOR & FRANCIS INC
Issue Date
2020-02
Language
English
Article Type
Article
Citation

RNA BIOLOGY, v.17, no.2, pp.176 - 187

ISSN
1547-6286
DOI
10.1080/15476286.2019.1672514
URI
http://hdl.handle.net/10203/271983
Appears in Collection
CH-Journal Papers(저널논문)
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