Micropipette Resonator Enabling Targeted Aspiration and Mass Measurement of Single Particles and Cells

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This paper reports micropipette resonators, mechanical resonator-integrated micropipettes, which enable selective aspiration and mass measurement of particles or cells suspended in liquids with two orthogonal vibration modes. A custom pipette pulling system is built to provide power-modulated linear heating on a rotating glass capillary to make an asymmetric cross section with extended uniformity.A glass capillary is stretched with the custom puller, cut within the pulled region, polished, mounted on a machined metallic jig, and then coated with a metal. As a result, a doubly clamped tube resonator-integrated micropipette is made. For simultaneous frequency readouts of two orthogonal modes, an optical pickup, originally developed for optical data storage, is configured closely above and properly aligned to the micropipette resonator and two digital phase-locked loops are, employed. For mass responsivity calibration, frequency shifts of the micropipette resonator are measured with various liquids and glass microparticles. Buoyant masses of unicellular organisms, Paramecium aurelia, freely swimming in a culture dish are successfully measured with two orthogonal modes.
Publisher
AMER CHEMICAL SOC
Issue Date
2019-12
Language
English
Article Type
Article
Citation

ACS SENSORS, v.4, no.12, pp.3275 - 3282

ISSN
2379-3694
DOI
10.1021/acssensors.9b01843
URI
http://hdl.handle.net/10203/271591
Appears in Collection
ME-Journal Papers(저널논문)
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