DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Minjeong | ko |
dc.contributor.author | Choi, Giwoong | ko |
dc.contributor.author | Choi, Yoon Young | ko |
dc.contributor.author | Lee, Jae Eun | ko |
dc.contributor.author | Jung, Jik-Han | ko |
dc.contributor.author | Oh, Seung Won | ko |
dc.contributor.author | Han, Dai Hoon | ko |
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Park, Ji-Ho | ko |
dc.contributor.author | Cheong, Jae-Ho | ko |
dc.contributor.author | Kim, Pilnam | ko |
dc.date.accessioned | 2019-12-31T01:20:23Z | - |
dc.date.available | 2019-12-31T01:20:23Z | - |
dc.date.created | 2019-12-30 | - |
dc.date.created | 2019-12-30 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | NPG ASIA MATERIALS, v.11 | - |
dc.identifier.issn | 1884-4049 | - |
dc.identifier.uri | http://hdl.handle.net/10203/270802 | - |
dc.description.abstract | Small extracellular vesicles (EVs), including exosomes, in body fluids have important applications in the noninvasive liquid biopsy-based diagnosis of cancer. Current EV-based diagnostic techniques still face practical challenges, such as inefficient EV isolation. Here, we report an efficient, resource-free pre-enrichment approach using (-)-epigallocatechin-3-gallate (EGCG), a polyphenolic biomolecule, to isolate and detect exosomal microRNAs (miRNAs) in human blood plasma samples. Our system comprises three steps: (1) EGCG-mediated EV aggregation, (2) filter-based EV isolation, and (3) molecular beacon-based detection of target miRNA in EVs. Using blood samples from cancer patients with gastric cancer or hepatocellular carcinoma, we constructed an EGCG-assisted miRNA diagnostic system. For both cancers, the levels of target miRNAs (miR-21, -27a, and -375) in EVs were strongly correlated with those in the publicly available GEO database. Our approach, an easy-to-use method for efficient EV isolation and the detection of miRNA in clinical samples, is applicable for molecular diagnostics in precision medicine. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Extracellular vesicle (EV)-polyphenol nanoaggregates for microRNA-based cancer diagnosis | - |
dc.type | Article | - |
dc.identifier.wosid | 000502984500003 | - |
dc.identifier.scopusid | 2-s2.0-85076373879 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.publicationname | NPG ASIA MATERIALS | - |
dc.identifier.doi | 10.1038/s41427-019-0184-0 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.localauthor | Park, Ji-Ho | - |
dc.contributor.localauthor | Kim, Pilnam | - |
dc.contributor.nonIdAuthor | Choi, Giwoong | - |
dc.contributor.nonIdAuthor | Choi, Yoon Young | - |
dc.contributor.nonIdAuthor | Lee, Jae Eun | - |
dc.contributor.nonIdAuthor | Oh, Seung Won | - |
dc.contributor.nonIdAuthor | Han, Dai Hoon | - |
dc.contributor.nonIdAuthor | Cheong, Jae-Ho | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | EXOSOME ISOLATION | - |
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