Extracellular vesicle (EV)-polyphenol nanoaggregates for microRNA-based cancer diagnosis

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Small extracellular vesicles (EVs), including exosomes, in body fluids have important applications in the noninvasive liquid biopsy-based diagnosis of cancer. Current EV-based diagnostic techniques still face practical challenges, such as inefficient EV isolation. Here, we report an efficient, resource-free pre-enrichment approach using (-)-epigallocatechin-3-gallate (EGCG), a polyphenolic biomolecule, to isolate and detect exosomal microRNAs (miRNAs) in human blood plasma samples. Our system comprises three steps: (1) EGCG-mediated EV aggregation, (2) filter-based EV isolation, and (3) molecular beacon-based detection of target miRNA in EVs. Using blood samples from cancer patients with gastric cancer or hepatocellular carcinoma, we constructed an EGCG-assisted miRNA diagnostic system. For both cancers, the levels of target miRNAs (miR-21, -27a, and -375) in EVs were strongly correlated with those in the publicly available GEO database. Our approach, an easy-to-use method for efficient EV isolation and the detection of miRNA in clinical samples, is applicable for molecular diagnostics in precision medicine.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2019-12
Language
English
Article Type
Article
Citation

NPG ASIA MATERIALS, v.11

ISSN
1884-4049
DOI
10.1038/s41427-019-0184-0
URI
http://hdl.handle.net/10203/270802
Appears in Collection
CH-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
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