Plant regeneration involves critical checkpoints including pluripotency acquisition and de novo organogenesis. However, comprehensive understanding of the mechanisms that underlie plant regeneration remains limited. Here, we found that calli derived from jasmonate (JA)-pretreated hypocotyl explants exhibited increased rates of de novo shoot regeneration. In contrast, exogenous JA treatment during callus formation on CIM did not influence the plant regeneration process. The enhanced shoot regeneration was diminished in coi1-1 mutants, indicating that JA-pretreated explants potentiate shoot regeneration in a COI1-dependent manner. These results suggest that the JA-responsive COI1 protein likely contributes to plant regeneration efficiency via regulation of hormone-signaling crosstalk and/or cell proliferation.