Physicochemically Tuned Myofibroblasts for Wound Healing Strategy

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 73
  • Download : 2
Normal healing of skin wounds involves a complex interplay between many different cellular constituents, including keratinocytes, immune cells, fibroblasts, myofibroblasts, as well as extracellular matrices. Especially, fibroblasts play a critical role in regulating the immune response and matrix reconstruction by secreting many cytokines and matrix proteins. Myofibroblasts, which are differentiated form of fibroblasts, feature high cellular contractility and encourage the synthesis of matrix proteins to promote faster closure of the wounds. We focus on the functional characteristics of these myofibroblasts as the healing strategy for severe wounds where the surplus amount of matrix proteins could be beneficial for better regeneration. In this study, we first employed multiple physicochemical cues, namely topographical alignment, TGF-beta 1, and electrical field (EF), to induce differentiation of dermal fibroblasts into myofibroblasts, and to further activate the differentiated cells. We then used these cells in a mouse wound model to verify their potential as a transplantable substitute for the severe wound. Our results confirmed that physicochemically stimulated myofibroblasts promoted faster healing of the wound compared to the case with non-stimulated myofibroblasts through elevated matrix reconstruction in the mouse model. Conclusively, we propose the utilization of physicochemically tuned myofibroblasts as a novel strategy for promoting better healing of moderate to severe wounds.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2019-11
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.9

ISSN
2045-2322
DOI
10.1038/s41598-019-52523-9
URI
http://hdl.handle.net/10203/268843
Appears in Collection
ME-Journal Papers(저널논문)
Files in This Item
s41598-019-52523-9.pdf(3.39 MB)Download

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0