Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins

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Intracellular antibodies have become powerful tools for imaging, modulating and neutralizing endogenous target proteins. Here, we describe an optogenetically activated intracellular antibody (optobody) consisting of split antibody fragments and blue-light inducible heterodimerization domains. We expanded this optobody platform by generating several optobodies from previously developed intracellular antibodies, and demonstrated that photoactivation of gelsolin and beta 2-adrenergic receptor (beta 2AR) optobodies suppressed endogenous gelsolin activity and beta 2AR signaling, respectively.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2019-11
Language
English
Article Type
Article
Citation

NATURE METHODS, v.16, no.11, pp.1095 - +

ISSN
1548-7091
DOI
10.1038/s41592-019-0592-7
URI
http://hdl.handle.net/10203/268840
Appears in Collection
BS-Journal Papers(저널논문)
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