Metabolic engineering of corynebacterium glutamicum for production of glutaric acid

Abstract

Environmental problems including climate change are urging us to establish sustainable chemical industry that produces chemicals, fuels and materials from renewable biomass using eco-friendly methods. The C5 platform chemicals including 5-aminovaleric acid (AVA) and glutaric acid can be used to produce valerolactam, 5-hydroxyvalerate and 1,5-pentanediol all in which can be used in turn for polyester, polyamide and polyol manufacture. The industrial strain, C. glutamicum, is widely used for global L-lysine, L-gluatmate and cadaverine market demand. Here, an efficient method to produce glutaric acid production by metabolic engineering of C. glutamicum is presented. The L-lysine biosynthetic pathway was extended by heterologous expression of L-lysine catabolic pathway to produce 100.22 g/L glutaric acid in a laboratory-scale bioreactor.