In this thesis, a simple method for constructing spheroid-hydrogel array chip using droplet contact-based transfer was demonstrated. When the upper spheroid-containing drop was in contact with the lower liquid drop, the air/liquid interface disappeared at the merged surface and the spheroid settled down due to gravitational force. This method was applied to repetitive medium change and live/dead staining of spheroids cultured with the hanging drop method using a polydimethylsiloxane (PDMS)-based drop array chip (DAC) having an array of well structures and peripheral rims. To embed the spheroids into the collagen hydrogel drops, the pillar array chip (PAC) was contacted in advance with the spheroid-containing DAC. The contacted PAC had the spheroids trapped in small liquid drops whose volume were reduced to around 1/16 of the drops loaded in DAC. The spheroids were embedded in the collagen drops at once by contacting the spheroid-containing PAC and the collagen-loaded DAC. The spheroids embedded in DAC-PAC contacting method showed the slowest invasion rate, reflecting homogeneity in the concentration or distribution of collagen drops. Finally, the spheroid？collagen DAC was treated with multiple concentrations of doxorubicin and paclitaxel to demonstrate the applicability of spheroid-hydrogel array chip to anti-cancer drug assays.