Research on the construction of complex protein using an alpha helix fusion method and its application = 알파 헬릭스 융합 방법을 이용한 복합 단백질 제작과 응용에 관한 연구

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To accurately design and generate artificial proteins and protein complexes, the method which can stably and predictably connect protein components is required. In this research, we have shown that ‘alpha helix fusion method’ can rigidly and predictably connect protein components. The successful design of these novel fusion proteins was confirmed by X-ray crystallography. Repeat proteins such as ankyrin repeats or leucine-rich repeat (LRR) proteins have multiple copies of repeating units. They have applications in protein engineering and drug design, and we could generate several repeat proteins which are highly similar to the shape of ankyrin or LRR proteins. Therefore, ‘alpha helix fusion method’ can be used to develop novel proteins mimicking the structure or function of biologically and therapeutically important natural proteins. For single particle analysis using cryo-EM, molecular weight of protein needs to be higher than 100 kDa, and existence of symmetry at the target protein is helpful. By using the alpha helix fusion method, we could generate tetrameric protein complex. Since its high molecular weight (>200 kDa) and twofold symmetry, this tetrameric complex generated by the alpha helix fusion method may be a convenient tool for the cryo-EM analysis of small proteins.
Advisors
Lee, Jie-Ohresearcher이지오researcher
Description
한국과학기술원 :화학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 2017.2,[v, 47 p. :]

Keywords

Alpha helix▼afusion method▼arepeat protein▼aprotein complex▼acryo-EM; 알파 헬릭스▼a융합 방법▼a반복 단백질▼a단백질 복합체▼a극저온전자현미경

URI
http://hdl.handle.net/10203/265486
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=866988&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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