Identifying the protective immunity induced by herpes zoster DNA vaccination = 대상포진 DNA 백신에 의해 유도된 방어 면역 형성 연구

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Herpes zoster (HZ), or shingles, is caused by the reactivation of latent varicella-zoster virus (VZV) from the sensory ganglia when VZV-specific T-cell immunity is decreased because of aging or immunosuppression. In the present study, we developed HZ DNA vaccine candidates encoding VZV proteins and cytokine adjuvants, such as IL-7 and IL-33. We immunized C57BL/6 mice with DNA plasmids encoding VZV glycoprotein E (gE), immediate early 63 (IE63), or immediate early 62 (IE62) proteins and found that robust VZV protein-specific T-cell responses were elicited by HZ DNA vaccination. Co-administration of DNA plasmids encoding IL-7 or IL-33 in HZ DNA vaccination significantly enhanced the magnitude of VZV protein-specific T-cell responses. Protective immunity elicited by HZ DNA vaccination was proven by challenge experiments with a surrogate virus, vaccinia virus expressing gE (VV-gE). A single dose of HZ DNA vaccine strongly boosted gE-specific T-cell responses in mice with a history of previous infection by VV-gE. Thus, HZ DNA vaccines with IL-7 and IL-33 adjuvants strongly elicit protective immunity.
Advisors
Shin, Eui-Cheolresearcher신의철researcher
Description
한국과학기술원 :의과학학제전공,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 의과학학제전공, 2019.2,[iv, 50 p. :]

Keywords

Herpes zoster▼aT cell▼aDNA vaccine▼aIL-7▼aIL-33; 대상 포진▼aT 세포▼aDNA 백신▼a인터루킨-7▼a인터루킨-33

URI
http://hdl.handle.net/10203/265117
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=842204&flag=dissertation
Appears in Collection
MSE-Theses_Ph.D.(박사논문)
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