DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Song, Ji-Joon | - |
dc.contributor.advisor | 송지준 | - |
dc.contributor.author | Yoon, Jungmin | - |
dc.date.accessioned | 2019-08-22T02:43:54Z | - |
dc.date.available | 2019-08-22T02:43:54Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=827902&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/264791 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2018.8,[iii, 84 p. :] | - |
dc.description.abstract | Importin4 transports histone H3/H4 in complex with Asf1a to the nucleus for chromatin assembly. Importin4 recognizes the nuclear localization sequence located at the N-terminal tail of histones. Here, we analyzed the structures and interactions of human Importin4, histones and Asf1a by cross-linking mass spectrometry (XL-MS), X-ray crystallography, negative-stain electron microscopy, small-angle X-ray scattering and integrative modeling. The XL-MS data showed that the C-terminal region of Importin4 was extensively crosslinked with the histone H3 tail. We determined the crystal structure of the C-terminal region of Importin4 bound to the histone H3 peptide, thus revealing that the acidic patch in Importin4 accommodates the histone H3 tail, and that histone H3 Lys14 contributes to the interaction with Importin4. In addition, we show that Asf1a modulates the binding of histone H3/H4 to Importin4. Furthermore, the molecular architecture of the Importin4_histone H3/H4_Asf1a complex was produced through an integrative modeling approach. Overall, this work provides structural insights into how Importin4 recognizes histones and their chaperone complex. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | chromatin▼anucleosome▼aassembly▼anuclear localization sequence▼aX-ray crystallography▼across-linking mass spectrometry▼aelectron microscopy▼asmall-angle x-ray scattering▼aintegrative structural modeling | - |
dc.subject | 염색질▼a핵산▼a조립▼a핵 배치서열▼a가교결합-질량분석기▼a전자현미경▼a소각 x-선 산란▼a통합적 구조 모델링 | - |
dc.title | Integrative structural investigation on human importin4_Histone H3/H4_Asf1a complex | - |
dc.title.alternative | Importin4_Histone H3/H4_Asf1a 복합체의 통합적 구조 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | 윤정민 | - |
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