DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Hyowon | ko |
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Lee, Seoyoung | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2019-07-03T07:30:05Z | - |
dc.date.available | 2019-07-03T07:30:05Z | - |
dc.date.created | 2019-03-11 | - |
dc.date.created | 2019-03-11 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | NANOSCALE, v.11, no.8, pp.3633 - 3638 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | http://hdl.handle.net/10203/262917 | - |
dc.description.abstract | A new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target nucleic acids. In the FERA method, flap endonuclease (FEN) catalyzesthe hydrolytic cleavage at the junction of single-and double-stranded DNAs which is formed only in the presence of target nucleic acids, and releases short oligonucleotides to promote the cyclic enzymatic repairing amplification (ERA) combined with FEN-based amplification. As a result, a large amount of single-and double-stranded DNAs are generated under the isothermal conditions, leading to the high fluorescence intensity from the SYBR I green dye. Relying on the powerful amplification method, we successfully determined the target nucleic acids with a limit of detection as low as 15.16 aM, which corresponds to approximately 180 molecules in 20 mu L reaction volume, and verified the practical applicability by detecting long target nucleic acids derived from Chlamydia trachomatis. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids | - |
dc.type | Article | - |
dc.identifier.wosid | 000459504400019 | - |
dc.identifier.scopusid | 2-s2.0-85061974788 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 3633 | - |
dc.citation.endingpage | 3638 | - |
dc.citation.publicationname | NANOSCALE | - |
dc.identifier.doi | 10.1039/c8nr06699j | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | STRAND DISPLACEMENT AMPLIFICATION | - |
dc.subject.keywordPlus | SEQUENCE REPLICATION 3SR | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | CIRCULATING DNA | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | POLYMERASE | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | STRATEGY | - |
dc.subject.keywordPlus | NASBA | - |
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