Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source

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dc.contributor.authorLee, Kyungsooko
dc.contributor.authorLee, Yong Jaeko
dc.contributor.authorChang, Ho Namko
dc.contributor.authorJeong, Ki Junko
dc.date.accessioned2019-06-24T08:30:02Z-
dc.date.available2019-06-24T08:30:02Z-
dc.date.created2019-06-24-
dc.date.created2019-06-24-
dc.date.issued2019-06-
dc.identifier.citationKOREAN JOURNAL OF CHEMICAL ENGINEERING, v.36, no.6, pp.903 - 908-
dc.identifier.issn0256-1115-
dc.identifier.urihttp://hdl.handle.net/10203/262809-
dc.description.abstractTrichosporon oleaginosus is one of the most promising hosts for microbial lipid production owing to its high-productivity; In an effort to develop an economical production process, we engineered T. oleaginosus towards high-lipid production from volatile fatty acids (VFA) derived from anaerobic fermentation of food waste. First, we established a method for labeling intracellular lipid with lipophilic BODIPY fluorescent dye. Next, a random library was constructed by treatment with a chemical mutagen, and high-lipid producers were screened using fluorescence-activated cell sorting. Subsequently, one clone, N14, was successfully isolated, which exhibited 3-fold higher lipid production (19.4%) in VFA (6 g/L) media than the wild-type strain, and also showed increased lipid production in higher concentrations of VFA (18 or 24 g/L). Based on fatty acid methyl ester (FAME) analysis, N14 contained higher stearic acid (C18:0) and oleic acid (C18:1) content compared with those of the wild-type strain.-
dc.languageEnglish-
dc.publisherKOREAN INSTITUTE CHEMICAL ENGINEERS-
dc.titleEngineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source-
dc.typeArticle-
dc.identifier.wosid000470736600009-
dc.identifier.scopusid2-s2.0-85061258929-
dc.type.rimsART-
dc.citation.volume36-
dc.citation.issue6-
dc.citation.beginningpage903-
dc.citation.endingpage908-
dc.citation.publicationnameKOREAN JOURNAL OF CHEMICAL ENGINEERING-
dc.identifier.doi10.1007/s11814-018-0229-7-
dc.identifier.kciidART002466632-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.nonIdAuthorLee, Kyungsoo-
dc.contributor.nonIdAuthorLee, Yong Jae-
dc.contributor.nonIdAuthorChang, Ho Nam-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorTrichosporon oleaginosus-
dc.subject.keywordAuthorVolatile Fatty Acids-
dc.subject.keywordAuthorFluorescence-activated Cell Sorting-
dc.subject.keywordAuthorMicrobial Lipid-
dc.subject.keywordAuthorBODIPY-
dc.subject.keywordPlusYEAST CRYPTOCOCCUS-CURVATUS-
dc.subject.keywordPlusMICROBIAL LIPIDS-
dc.subject.keywordPlusBIOCONVERSION-
dc.subject.keywordPlusFERMENTATION-
dc.subject.keywordPlusWASTE-
dc.subject.keywordPlusOIL-
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