DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kyungsoo | ko |
dc.contributor.author | Lee, Yong Jae | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2019-06-24T08:30:02Z | - |
dc.date.available | 2019-06-24T08:30:02Z | - |
dc.date.created | 2019-06-24 | - |
dc.date.created | 2019-06-24 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.citation | KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.36, no.6, pp.903 - 908 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.uri | http://hdl.handle.net/10203/262809 | - |
dc.description.abstract | Trichosporon oleaginosus is one of the most promising hosts for microbial lipid production owing to its high-productivity; In an effort to develop an economical production process, we engineered T. oleaginosus towards high-lipid production from volatile fatty acids (VFA) derived from anaerobic fermentation of food waste. First, we established a method for labeling intracellular lipid with lipophilic BODIPY fluorescent dye. Next, a random library was constructed by treatment with a chemical mutagen, and high-lipid producers were screened using fluorescence-activated cell sorting. Subsequently, one clone, N14, was successfully isolated, which exhibited 3-fold higher lipid production (19.4%) in VFA (6 g/L) media than the wild-type strain, and also showed increased lipid production in higher concentrations of VFA (18 or 24 g/L). Based on fatty acid methyl ester (FAME) analysis, N14 contained higher stearic acid (C18:0) and oleic acid (C18:1) content compared with those of the wild-type strain. | - |
dc.language | English | - |
dc.publisher | KOREAN INSTITUTE CHEMICAL ENGINEERS | - |
dc.title | Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source | - |
dc.type | Article | - |
dc.identifier.wosid | 000470736600009 | - |
dc.identifier.scopusid | 2-s2.0-85061258929 | - |
dc.type.rims | ART | - |
dc.citation.volume | 36 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 903 | - |
dc.citation.endingpage | 908 | - |
dc.citation.publicationname | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.identifier.doi | 10.1007/s11814-018-0229-7 | - |
dc.identifier.kciid | ART002466632 | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Lee, Kyungsoo | - |
dc.contributor.nonIdAuthor | Lee, Yong Jae | - |
dc.contributor.nonIdAuthor | Chang, Ho Nam | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Trichosporon oleaginosus | - |
dc.subject.keywordAuthor | Volatile Fatty Acids | - |
dc.subject.keywordAuthor | Fluorescence-activated Cell Sorting | - |
dc.subject.keywordAuthor | Microbial Lipid | - |
dc.subject.keywordAuthor | BODIPY | - |
dc.subject.keywordPlus | YEAST CRYPTOCOCCUS-CURVATUS | - |
dc.subject.keywordPlus | MICROBIAL LIPIDS | - |
dc.subject.keywordPlus | BIOCONVERSION | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | WASTE | - |
dc.subject.keywordPlus | OIL | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.