Mechanisms for Hfq-Independent Activation of rpoS by DsrA, a Small RNA, in Escherichia coli

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dc.contributor.authorKim, Wonkyongko
dc.contributor.authorChoi, Jee Sooko
dc.contributor.authorKim, Daunko
dc.contributor.authorShin, Doohangko
dc.contributor.authorSuk, Shinaeko
dc.contributor.authorLee, Younghoonko
dc.date.accessioned2019-06-17T05:10:05Z-
dc.date.available2019-06-17T05:10:05Z-
dc.date.created2019-06-12-
dc.date.created2019-06-12-
dc.date.created2019-06-12-
dc.date.created2019-06-12-
dc.date.issued2019-05-
dc.identifier.citationMOLECULES AND CELLS, v.42, no.5, pp.426 - 439-
dc.identifier.issn1016-8478-
dc.identifier.urihttp://hdl.handle.net/10203/262613-
dc.description.abstractMany small RNAs (sRNAs) regulate gene expression by base pairing to their target messenger RNAs (mRNAs) with the help of Hfq in Escherichia coli. The sRNA DsrA activates translation of the rpoS mRNA in an Hfq-dependent manner, but this activation ability was found to partially bypass Hfq when DsrA is overproduced. The precise mechanism by which DsrA bypasses Hfq is unknown. In this study, we constructed strains lacking all three rpoS-activating sRNAs (i.e., ArcZ, DsrA, and RprA) in hfq(+) and Hfq(-) backgrounds, and then artificially regulated the cellular DsrA concentration in these strains by controlling its ectopic expression. We then examined how the expression level of rpoS was altered by a change in the concentration of DsrA. We found that the translation and stability of the rpoS mRNA are both enhanced by physiological concentrations of DsrA regardless of Hfq, but that depletion of Hfq causes a rapid degradation of DsrA and thereby decreases rpoS mRNA stability. These results suggest that the observed Hfq dependency of DsrA-mediated rpoS activation mainly results from the destabilization of DsrA in the absence of Hfq, and that DsrA itself contributes to the translational activation and stability of the rpoS mRNA in an Hfq-independent manner.-
dc.languageEnglish-
dc.publisherKOREAN SOC MOLECULAR & CELLULAR BIOLOGY-
dc.titleMechanisms for Hfq-Independent Activation of rpoS by DsrA, a Small RNA, in Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000468918600006-
dc.identifier.scopusid2-s2.0-85066920257-
dc.type.rimsART-
dc.citation.volume42-
dc.citation.issue5-
dc.citation.beginningpage426-
dc.citation.endingpage439-
dc.citation.publicationnameMOLECULES AND CELLS-
dc.identifier.doi10.14348/molcells.2019.0040-
dc.identifier.kciidART002466949-
dc.contributor.localauthorLee, Younghoon-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorDsrA-
dc.subject.keywordAuthorEscherichia coli-
dc.subject.keywordAuthorHfq-
dc.subject.keywordAuthorrpoS-
dc.subject.keywordAuthorsmall RNAs-
dc.subject.keywordPlusSM-LIKE PROTEIN-
dc.subject.keywordPlusMESSENGER-RNA-
dc.subject.keywordPlusREGULATORY RNAS-
dc.subject.keywordPlusTRANSLATIONAL ACTIVATION-
dc.subject.keywordPlusINTERACTION SURFACES-
dc.subject.keywordPlusPOSITIVE REGULATION-
dc.subject.keywordPlusDUPLEX FORMATION-
dc.subject.keywordPlusSOLUBLE-RNAS-
dc.subject.keywordPlusBINDING-
dc.subject.keywordPlusRIBOREGULATION-
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CH-Journal Papers(저널논문)
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