DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Myung Hee | ko |
dc.contributor.author | Kang, Jung-Ok | ko |
dc.contributor.author | Kim, Joo-Young | ko |
dc.contributor.author | Jun, Hi Eun | ko |
dc.contributor.author | Lee, Heung Kyu | ko |
dc.contributor.author | Chang, Jun | ko |
dc.date.accessioned | 2019-04-15T14:30:58Z | - |
dc.date.available | 2019-04-15T14:30:58Z | - |
dc.date.created | 2019-03-18 | - |
dc.date.created | 2019-03-18 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | ANTIVIRAL RESEARCH, v.163, pp.19 - 28 | - |
dc.identifier.issn | 0166-3542 | - |
dc.identifier.uri | http://hdl.handle.net/10203/254124 | - |
dc.description.abstract | Nucleoprotein is highly conserved among each type of influenza viruses (A and B) and has received significant attention as a good target for universal influenza vaccine. In this study, we determined whether a recombinant adenovirus encoding nucleoprotein of type B influenza virus (rAd/B-NP) confers protection against influenza virus infection in mice. We also identified a cytotoxic T lymphocyte epitope in the nucleoprotein to determine B-NP-specific CD8 T-cell responses. We found that B-NP-specific CD8 T cells induced by rAd/B-NP immunization played a major role in protection following influenza B virus infection using CD8 knockout mice. To assess the effects of the administration routes on protective immunity, we immunized mice with rAd/B-NP via intranasal or intramuscular routes. Both groups showed strong NP-specific humoral and CD8 T-cell responses, but only intranasal immunization provided complete protection against both lineages of influenza B virus challenge. Intranasal but not intramuscular administration established resident memory CD8 T cells in the airway and lung parenchyma, which were required for efficient protection. Furthermore, rAd/B-NP in combination with rAd/A-NP protected mice against lethal infection with both influenza A and B viruses. These findings demonstrate that rAd/B-NP could be further developed as a universal vaccine against influenza. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Single mucosal vaccination targeting nucleoprotein provides broad protection against two lineages of influenza B virus | - |
dc.type | Article | - |
dc.identifier.wosid | 000459843400003 | - |
dc.identifier.scopusid | 2-s2.0-85059850636 | - |
dc.type.rims | ART | - |
dc.citation.volume | 163 | - |
dc.citation.beginningpage | 19 | - |
dc.citation.endingpage | 28 | - |
dc.citation.publicationname | ANTIVIRAL RESEARCH | - |
dc.identifier.doi | 10.1016/j.antiviral.2019.01.002 | - |
dc.contributor.localauthor | Lee, Heung Kyu | - |
dc.contributor.nonIdAuthor | Kim, Myung Hee | - |
dc.contributor.nonIdAuthor | Kang, Jung-Ok | - |
dc.contributor.nonIdAuthor | Kim, Joo-Young | - |
dc.contributor.nonIdAuthor | Jun, Hi Eun | - |
dc.contributor.nonIdAuthor | Chang, Jun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Influenza B virus | - |
dc.subject.keywordAuthor | Vaccine | - |
dc.subject.keywordAuthor | Nucleoprotein | - |
dc.subject.keywordAuthor | Mucosal immunization | - |
dc.subject.keywordAuthor | Protection | - |
dc.subject.keywordPlus | MEMORY T-CELLS | - |
dc.subject.keywordPlus | RESPIRATORY-TRACT | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordPlus | ROUTE | - |
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