DNA repair pathways are closely associated with the maintenance of genomic integrity, disease outbreak, and the development of therapeutics. Owing to these significances, novel analytical methods for enzymes that are involved in the DNA repair pathways have been actively investigated. This review focuses on discussions on nucleic acid-based methods, especially those based on the fluorescence, for the determination of DNA repair enzymes. Furthermore, this review not only provides meaningful insights in creating ingenious fluorescent detection methods but it also suggests future directions that will be followed in developing new analytical technologies for the DNA repair enzymes. (C) 2019 Elsevier B.V. All rights reserved.