Lissajous Scanning Two-photon Endomicroscope for in vivo Tissue Imaging

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dc.contributor.authorKim, Daniel Youngsukko
dc.contributor.authorHwang, Kyungminko
dc.contributor.authorAhn, Jinhyoko
dc.contributor.authorSeo, Yeong-Hyeonko
dc.contributor.authorKim, Jae-Beomko
dc.contributor.authorLee, Soyoungko
dc.contributor.authorYoon, Jin Huiko
dc.contributor.authorKong, Eunjiko
dc.contributor.authorJeong, Yongko
dc.contributor.authorJon, Sangyongko
dc.contributor.authorKim, Pilhanko
dc.contributor.authorJeong, Ki-Hunko
dc.date.accessioned2019-03-19T01:50:19Z-
dc.date.available2019-03-19T01:50:19Z-
dc.date.created2019-03-06-
dc.date.created2019-03-06-
dc.date.created2019-03-06-
dc.date.created2019-03-06-
dc.date.created2019-03-06-
dc.date.created2019-03-06-
dc.date.issued2019-03-
dc.identifier.citationSCIENTIFIC REPORTS, v.9, pp.3560-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10203/251784-
dc.description.abstractAn endomicroscope opens new frontiers of non-invasive biopsy for in vivo imaging applications. Here we report two-photon laser scanning endomicroscope for in vivo cellular and tissue imaging using a Lissajous fiber scanner. The fiber scanner consists of a piezoelectric (PZT) tube, a single double-clad fiber (DCF) with high fluorescence collection, and a micro-tethered-silicon-oscillator (MTSO) for the separation of biaxial resonant scanning frequencies. The endomicroscopic imaging exhibits 5 frames/s with 99% in scanning density by using the selection rule of scanning frequencies. The endomicroscopic scanner was compactly packaged within a stainless tube of 2.6 mm in diameter with a high NA gradient-index (GRIN) lens, which can be easily inserted into the working channel of a conventional laparoscope. The lateral and axial resolutions of the endomicroscope are 0.70 µm and 7.6 μm, respectively. Two-photon fluorescence images of a stained kidney section and miscellaneous ex vivo and in vivo organs from wild type and green fluorescent protein transgenic (GFP-TG) mice were successfully obtained by using the endomicroscope. The endomicroscope also obtained label free images including autofluorescence and second-harmonic generation of an ear tissue of Thy1-GCaMP6 (GP5.17) mouse. The Lissajous scanning two-photon endomicroscope can provide a compact handheld platform for in vivo tissue imaging or optical biopsy applications.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleLissajous Scanning Two-photon Endomicroscope for in vivo Tissue Imaging-
dc.typeArticle-
dc.identifier.wosid000460381600133-
dc.identifier.scopusid2-s2.0-85062586761-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.beginningpage3560-
dc.citation.publicationnameSCIENTIFIC REPORTS-
dc.identifier.doi10.1038/s41598-019-38762-w-
dc.contributor.localauthorJeong, Yong-
dc.contributor.localauthorJon, Sangyong-
dc.contributor.localauthorKim, Pilhan-
dc.contributor.localauthorJeong, Ki-Hun-
dc.contributor.nonIdAuthorKim, Daniel Youngsuk-
dc.contributor.nonIdAuthorLee, Soyoung-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusOPTICAL COHERENCE TOMOGRAPHY-
dc.subject.keywordPlusFIBER-
dc.subject.keywordPlusMICROSCOPE-
dc.subject.keywordPlusENDOSCOPY-
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