DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Daesun | ko |
dc.contributor.author | Jung, Yongwon | ko |
dc.date.accessioned | 2019-03-19T01:25:30Z | - |
dc.date.available | 2019-03-19T01:25:30Z | - |
dc.date.created | 2019-03-04 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.58, no.7, pp.2045 - 2049 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/251625 | - |
dc.description.abstract | Cell surface receptor oligomerization is an attractive target process for drug screening. However, simple but reliable (and thus high-throughput) visualization methods for receptor oligomerization are still lacking. Herein, we report on a new single-construct homo-molecular fluorescence complementation (Homo-FC) probe, which shows strong fluorescence signals by oligomerization of fused receptors in living cells with unexpectedly low background signals. Importantly, this high signal-to-noise ratio was not affected by expression level variations of fused receptors. The Homo-FC probe was developed by optimized flopped fusion of split fragments of superfolder green fluorescence protein and subsequent surface charge engineering. Homo-FC reliably visualized the oligomerization of diverse natural receptors such as GPCR, EGFR, and even cytosolic DAI. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Homo-molecular Fluorescence Complementation for Direct Visualization of Receptor Oligomerization in Living Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000458828000028 | - |
dc.identifier.scopusid | 2-s2.0-85060094290 | - |
dc.type.rims | ART | - |
dc.citation.volume | 58 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 2045 | - |
dc.citation.endingpage | 2049 | - |
dc.citation.publicationname | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | - |
dc.identifier.doi | 10.1002/anie.201812780 | - |
dc.contributor.localauthor | Jung, Yongwon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | drug discovery | - |
dc.subject.keywordAuthor | fluorescence complementation | - |
dc.subject.keywordAuthor | imaging | - |
dc.subject.keywordAuthor | membrane receptor oligomerization | - |
dc.subject.keywordAuthor | protein engineering | - |
dc.subject.keywordPlus | PROTEINS | - |
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