Homo-molecular Fluorescence Complementation for Direct Visualization of Receptor Oligomerization in Living Cells

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Cell surface receptor oligomerization is an attractive target process for drug screening. However, simple but reliable (and thus high-throughput) visualization methods for receptor oligomerization are still lacking. Herein, we report on a new single-construct homo-molecular fluorescence complementation (Homo-FC) probe, which shows strong fluorescence signals by oligomerization of fused receptors in living cells with unexpectedly low background signals. Importantly, this high signal-to-noise ratio was not affected by expression level variations of fused receptors. The Homo-FC probe was developed by optimized flopped fusion of split fragments of superfolder green fluorescence protein and subsequent surface charge engineering. Homo-FC reliably visualized the oligomerization of diverse natural receptors such as GPCR, EGFR, and even cytosolic DAI.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2019-02
Language
English
Article Type
Article
Citation

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.58, no.7, pp.2045 - 2049

ISSN
1433-7851
DOI
10.1002/anie.201812780
URI
http://hdl.handle.net/10203/251625
Appears in Collection
CH-Journal Papers(저널논문)
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