DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeon Chul | - |
dc.contributor.author | Kwon, Sunhoon | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.contributor.author | Chang, Ho Nam | - |
dc.date.accessioned | 2011-09-09T05:16:00Z | - |
dc.date.available | 2011-09-09T05:16:00Z | - |
dc.date.issued | 1998-08 | - |
dc.identifier.citation | Biotechnology Letters, Vol.20, No.8, pp.799-803 | en |
dc.identifier.uri | http://hdl.handle.net/10203/25160 | - |
dc.description.abstract | In batch cultivation, growth of a recombinant Escherichia coli with an inducible T7 expression system and maximum expression of a bioadhesive precursor (BP) protein was similar in the strains with and without the plasmid vector, pLysS. In fed-batch cultivation, however, the strain harboring pLysS grew slower and had a lower level of BP protein expression than that obtained with the strain without pLysS. This suggests that the presence of pLysS in the T7 expression system strongly affects the cell growth and expression of BP protein in high cell density cultivation. | en |
dc.description.sponsorship | The authors thank Dr.Ina Goldberg for providing the E.coli AS002(pAG9). This work was suppported by the BioProcess Engineering Research Center and by the Ministry of Science and Technology. | en |
dc.language.iso | en_US | en |
dc.publisher | Springer Verlag | en |
dc.title | Improved production of a bioadhesive precursor protein by fed-batch cultivation of a recombinant Escherichia coli with a pLysS vector | en |
dc.type | Article | en |
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