A hemicyanine-embedded diphenylselenide-containing probe “HemiSe” in which SePh2 stays reduced for selective detection of superoxide in living cells

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dc.contributor.authorHalle, Mahesh B.ko
dc.contributor.authorLee, KyungJinko
dc.contributor.authorYudhistira, Teslako
dc.contributor.authorChoi, Jaehyuckko
dc.contributor.authorPark, Hee-Sungko
dc.contributor.authorChurchill, David Gko
dc.date.accessioned2019-01-23T06:19:43Z-
dc.date.available2019-01-23T06:19:43Z-
dc.date.created2018-10-17-
dc.date.issued2018-11-
dc.identifier.citationCHEMISTRY-AN ASIAN JOURNAL, v.13, no.24, pp.3895 - 3902-
dc.identifier.issn1861-4728-
dc.identifier.urihttp://hdl.handle.net/10203/250020-
dc.description.abstractA simple one‐step synthesis of fluorescent probe HemiSe has been developed for the detection of superoxide (O2.−). The probe undergoes reaction specifically with O2.− when in the presence of other competitive ROS/RNS/metal ions. The diphenylselenide was incorporated to completely quench the fluorescence of the hemicyanine unit through the action of a photoinduced electron transfer (PET) photomechanism. However, after the addition of O2.−, the latent fluorophore regains its fluorescence owing to the reaction at the C=C bond of the hemicyanine with O2.− through nucleophilic attack; the increase in blue emission is due to a reaction of the double bond within HemiSe followed by an increase in fluorescence quantum yield (Φ) up to 0.45; the limit of detection (LOD) is 11.9 nm. A time‐dependent study shows that HemiSe can detect superoxide within 13 min with high sensitivity, high selectivity, over a wide pH range, and through confirmation with a xanthine/xanthine oxidase biochemical assay (λem=439 nm). A study in the RAW 264.7 macrophage living cells also shows that HemiSe is not toxic, cell permeable (experimental log P=2.11); confocal imaging results show that HemiSe can detect O2.− in endogenous and exogeneous systems.-
dc.languageEnglish-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.titleA hemicyanine-embedded diphenylselenide-containing probe “HemiSe” in which SePh2 stays reduced for selective detection of superoxide in living cells-
dc.typeArticle-
dc.identifier.wosid000453745000014-
dc.identifier.scopusid2-s2.0-85056458051-
dc.type.rimsART-
dc.citation.volume13-
dc.citation.issue24-
dc.citation.beginningpage3895-
dc.citation.endingpage3902-
dc.citation.publicationnameCHEMISTRY-AN ASIAN JOURNAL-
dc.identifier.doi10.1002/asia.201801339-
dc.contributor.localauthorPark, Hee-Sung-
dc.contributor.localauthorChurchill, David G-
dc.contributor.nonIdAuthorHalle, Mahesh B.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
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