DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyeongseok | ko |
dc.contributor.author | Kim, Yeong Gi | ko |
dc.contributor.author | Choi, Wonsuk | ko |
dc.contributor.author | Moon, Joon Ho | ko |
dc.contributor.author | Hwang, Inseon | ko |
dc.contributor.author | Kim, Kyu Ho | ko |
dc.contributor.author | Yadav, Vijay K. | ko |
dc.contributor.author | Karsenty, Gerard | ko |
dc.contributor.author | Jeong, Ji-Seon | ko |
dc.contributor.author | Kim, Hail | ko |
dc.date.accessioned | 2019-01-22T08:57:52Z | - |
dc.date.available | 2019-01-22T08:57:52Z | - |
dc.date.created | 2018-12-31 | - |
dc.date.created | 2018-12-31 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.8 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/249161 | - |
dc.description.abstract | Recent studies on tissue-autonomous serotonin (5-hydroxytryptamine [5-HT]) function have identified new roles for 5-HT in peripheral organs. Most of these studies were performed by crossing mice carrying the Tph1(tm1Kry) allele with tissue specific Cre mice. In the present study, we found that 5-HT production was not completely abolished in Tph1(tm1Kry) KO mice. The residual 5-HT production in Tph1(tm1Kry) KO mice is attributed to the expression of a truncated form of TPH1 containing the catalytic domain. Hence, in an effort to obtain mice with a Tph1 null phenotype, we generated mice harboring a new Tph1 floxed allele, Tph1(tm1c), targeting exons 5 and 6 which encode the catalytic domain of TPH1. By crossing the new Tph1 floxed mice with villin-Cre or insulin-Cre mice, we observed near-complete ablation of 5-HT production in the intestine and beta cells. In conclusion, this improved Tph1 floxed mouse model will serve as useful and accurate tool for analyzing peripheral 5-HT system. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Generation of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model | - |
dc.type | Article | - |
dc.identifier.wosid | 000452204400011 | - |
dc.identifier.scopusid | 2-s2.0-85057730137 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/s41598-018-36097-6 | - |
dc.contributor.localauthor | Kim, Hail | - |
dc.contributor.nonIdAuthor | Yadav, Vijay K. | - |
dc.contributor.nonIdAuthor | Karsenty, Gerard | - |
dc.contributor.nonIdAuthor | Jeong, Ji-Seon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PERIPHERAL SEROTONIN | - |
dc.subject.keywordPlus | INSULIN-SECRETION | - |
dc.subject.keywordPlus | MASS | - |
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