Generation of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model

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Recent studies on tissue-autonomous serotonin (5-hydroxytryptamine [5-HT]) function have identified new roles for 5-HT in peripheral organs. Most of these studies were performed by crossing mice carrying the Tph1(tm1Kry) allele with tissue specific Cre mice. In the present study, we found that 5-HT production was not completely abolished in Tph1(tm1Kry) KO mice. The residual 5-HT production in Tph1(tm1Kry) KO mice is attributed to the expression of a truncated form of TPH1 containing the catalytic domain. Hence, in an effort to obtain mice with a Tph1 null phenotype, we generated mice harboring a new Tph1 floxed allele, Tph1(tm1c), targeting exons 5 and 6 which encode the catalytic domain of TPH1. By crossing the new Tph1 floxed mice with villin-Cre or insulin-Cre mice, we observed near-complete ablation of 5-HT production in the intestine and beta cells. In conclusion, this improved Tph1 floxed mouse model will serve as useful and accurate tool for analyzing peripheral 5-HT system.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2018-12
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.8

ISSN
2045-2322
DOI
10.1038/s41598-018-36097-6
URI
http://hdl.handle.net/10203/249161
Appears in Collection
MSE-Journal Papers(저널논문)
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