Electric field-induced migration and intercellular stress alignment in a collective epithelial monolayer

Cited 6 time in webofscience Cited 0 time in scopus
  • Hit : 151
  • Download : 0
During wound healing, cells migrate with electrotactic bias as a collective entity. Unlike the case of the electric field (EF)-induced single-cell migration, the sensitivity of electrotactic response of the monolayer depends primarily on the integrity of the cell-cell junctions. Although there exist biochemical clues on how cells sense the EF, a well-defined physical portrait to illustrate how collective cells respond to directional EF remains elusive. Here, we developed an EF stimulating system integrated with a hydrogel-based traction measurement platform to quantify the EF-induced changes in cellular tractions, from which the complete in-plane intercellular stress tensor can be calculated. We chose immortalized human keratinocytes, HaCaT, as our model cells to investigate the role of EF in epithelial migration during wound healing. Immediately after the onset of EF (0.5 V/cm), the HaCaT monolayer migrated toward anode with ordered directedness and enhanced speed as early as 15 min. Cellular traction and intercellular stresses were gradually aligned perpendicular to the direction of the EF until 50 min. The EF-induced reorientation of physical stresses was then followed by the delayed cell-body reorientation in the direction perpendicular to the EF. Once the intercellular stresses were aligned, the reversal of the EF direction redirected the reversed migration of the cells without any apparent disruption of the intercellular stresses. The results suggest that the dislodging of the physical stress alignment along the adjacent cells should not be necessary for changing the direction of the monolayer migration. © 2018 Cho et al.
Publisher
AMER SOC CELL BIOLOGY
Issue Date
2018-09
Language
English
Article Type
Article
Citation

MOLECULAR BIOLOGY OF THE CELL, v.29, no.19, pp.2292 - 2302

ISSN
1059-1524
DOI
10.1091/mbc.E18-01-0077
URI
http://hdl.handle.net/10203/248761
Appears in Collection
ME-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 6 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0