DC Field | Value | Language |
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dc.contributor.author | Kim, Mi-Hee | ko |
dc.contributor.author | Na, Hee-Kyung | ko |
dc.contributor.author | Kim, Young-Kwan | ko |
dc.contributor.author | Ryoo, Soo-Ryoon | ko |
dc.contributor.author | Cho, Hae-Sung | ko |
dc.contributor.author | Lee, Kyung-Eun | ko |
dc.contributor.author | Jeon, Hye-Sung | ko |
dc.contributor.author | Ryoo, Ryong | ko |
dc.contributor.author | Min, Dal-Hee | ko |
dc.date.accessioned | 2011-07-28T01:59:48Z | - |
dc.date.available | 2011-07-28T01:59:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.citation | ACS NANO, v.5, no.5, pp.3568 - 3576 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/24714 | - |
dc.description.abstract | Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy preparation in large quantity with low cost. Here, we report on a facile synthesis of monodispersed mesoporous silica nanoparticles (MMSN) possessing very large pores (> 15 nm) and application of the nanoparticles to plasmid DNA delivery to human cells. The aminated MMSN with large pores provided a higher loading capacity for plasmids than those with small pores (similar to 2 nm), and the complex of MMSN with plasmid DNA readily entered Into cells without supplementary polymers such as cationic dendrimers. Furthermore, MMSN with large pores could efficiently protect plasmids from nuclease-mediated degradation and showed much higher transfection efficiency of the plasmids encoding luciferase and green fluorescent protein. (pLuc, pGFP) compared to MMSN with small pores (similar to 2 nm). | - |
dc.description.sponsorship | This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (Grant Nos. 313-2008-2-C00538, 2008-0062074), by the Nano R&D program of NRF funded by MEST (2008-2004457), and by the National Honor Scientist Program (20100029665) and World Class University Program (R31-2010-000-10071-0) of NRF funded by MEST. H. Jeon was partially supported by a GRL “Theragnosis” grant from the Korean Government (MEST) and thanks the Advanced Analysis Center in KIST (Korea Institute of Science & Technology) for use of the TEM. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | TRANSGENIC MOUSE MODELS | - |
dc.subject | SICKLE-CELL-DISEASE | - |
dc.subject | PLASMID DNA | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | THERAPY | - |
dc.subject | VECTORS | - |
dc.subject | SPHERES | - |
dc.title | Facile Synthesis of Monodispersed Mesoporous Silica Nanoparticles with Ultralarge Pores and Their Application in Gene Delivery | - |
dc.type | Article | - |
dc.identifier.wosid | 000290826800019 | - |
dc.identifier.scopusid | 2-s2.0-80051489934 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 3568 | - |
dc.citation.endingpage | 3576 | - |
dc.citation.publicationname | ACS NANO | - |
dc.identifier.doi | 10.1021/nn103130q | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Ryoo, Ryong | - |
dc.contributor.nonIdAuthor | Kim, Mi-Hee | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Eun | - |
dc.contributor.nonIdAuthor | Jeon, Hye-Sung | - |
dc.contributor.nonIdAuthor | Min, Dal-Hee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biocompatibility | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | large pores | - |
dc.subject.keywordAuthor | plasmid | - |
dc.subject.keywordAuthor | porous silica nanoparticle | - |
dc.subject.keywordPlus | TRANSGENIC MOUSE MODELS | - |
dc.subject.keywordPlus | SICKLE-CELL-DISEASE | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | VECTORS | - |
dc.subject.keywordPlus | SPHERES | - |
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