DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Hyundoo | ko |
dc.contributor.author | Kang, Gyumin | ko |
dc.contributor.author | Yeon, Ju Hun | ko |
dc.contributor.author | NAM, YOONKEY | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2011-07-26T07:50:58Z | - |
dc.date.available | 2011-07-26T07:50:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-01 | - |
dc.identifier.citation | LAB ON A CHIP, v.9, no.1, pp.167 - 170 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/24709 | - |
dc.description.abstract | The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 mu m feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful 'hands-on' tool for the generation of micro-patterned chemical or biological assays using cells and proteins. | - |
dc.description.sponsorship | This work was supported by the Korea Science and Engineering Foundation (KOSEF) NRL Program grant funded by the Korea government (MEST) (R0A-2008-000-20109-0), by the Nano/ BioScience and Technology Program (2005-01291) of the MEST, and by the System IC 2010 program (10030554) of the Ministry of Knowledge and Economy (MKE). The authors also thank the Chung Moon Soul Center for BioInformation and Bio- Electronics, KAIST. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000262649400024 | - |
dc.identifier.scopusid | 2-s2.0-57449105074 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 167 | - |
dc.citation.endingpage | 170 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/b810341k | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | NAM, YOONKEY | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SOFT LITHOGRAPHY | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | DEPOSITION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | MASTERS | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | SYSTEM | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.