Spatiotemporal Control of TGF-β Signaling with Light

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 315
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorYuchao Liko
dc.contributor.authorMinji Leeko
dc.contributor.authorNury Kimko
dc.contributor.authorGuoyu Wuko
dc.contributor.authorDifan Dengko
dc.contributor.authorJin Man Kimko
dc.contributor.authorXuedong Liuko
dc.contributor.authorHeo, Won Doko
dc.contributor.authorZhike Ziko
dc.date.accessioned2018-07-24T01:47:13Z-
dc.date.available2018-07-24T01:47:13Z-
dc.date.created2018-01-30-
dc.date.issued2018-06-
dc.identifier.citationACS SYNTHETIC BIOLOGY, v.7, no.2, pp.443 - 451-
dc.identifier.issn2161-5063-
dc.identifier.urihttp://hdl.handle.net/10203/243799-
dc.description.abstractCells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space. Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level. © XXXX American Chemical Society-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleSpatiotemporal Control of TGF-β Signaling with Light-
dc.typeArticle-
dc.type.rimsART-
dc.citation.volume7-
dc.citation.issue2-
dc.citation.beginningpage443-
dc.citation.endingpage451-
dc.citation.publicationnameACS SYNTHETIC BIOLOGY-
dc.identifier.doi10.1021/acssynbio.7b00225-
dc.contributor.localauthorHeo, Won Do-
dc.contributor.nonIdAuthorYuchao Li-
dc.contributor.nonIdAuthorNury Kim-
dc.contributor.nonIdAuthorGuoyu Wu-
dc.contributor.nonIdAuthorDifan Deng-
dc.contributor.nonIdAuthorJin Man Kim-
dc.contributor.nonIdAuthorXuedong Liu-
dc.contributor.nonIdAuthorZhike Zi-
dc.description.isOpenAccessN-
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0