DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jong Won | ko |
dc.contributor.author | Lee, Joong-Jae | ko |
dc.contributor.author | Choi, Joon Sig | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2018-07-24T01:37:33Z | - |
dc.date.available | 2018-07-24T01:37:33Z | - |
dc.date.created | 2018-06-25 | - |
dc.date.created | 2018-06-25 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.544, no.1, pp.39 - 45 | - |
dc.identifier.issn | 0378-5173 | - |
dc.identifier.uri | http://hdl.handle.net/10203/243691 | - |
dc.description.abstract | Although a variety of non-viral gene delivery systems have been developed, they still suffer from low efficiency and specificity. Herein, we present the assembly of a dendrimer complex comprising a DNA cargo and a targeting moiety as a new format for targeted gene delivery. A PAMAM dendrimer modified with histidine and arginine (HR-dendrimer) was used to enhance the endosomal escape and transfection efficiency. An EGFR-specific repebody, composed of leucine-rich repeat (LRR) modules, was employed as a targeting moiety. A polyanionic peptide was genetically fused to the repebody, followed by incubation with an HR-dendrimer and a DNA cargo to assemble the dendrimer complex through an electrostatic interaction. The resulting dendrimer complex was shown to deliver a DNA cargo with high efficiency in a receptor-specific manner. An analysis using a confocal microscope confirmed the internalization of the dendrimer complex and subsequent dissociation of a DNA cargo from the complex. The present approach can be broadly used in a targeted gene delivery in many areas. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | CANCER-THERAPY | - |
dc.subject | NANOPARTICLES | - |
dc.subject | POLYMERS | - |
dc.subject | DESIGN | - |
dc.subject | CELLS | - |
dc.subject | TUMOR | - |
dc.subject | DERIVATIVES | - |
dc.subject | EXPRESSION | - |
dc.subject | CARRIER | - |
dc.title | Electrostatically assembled dendrimer complex with a high-affinity protein binder for targeted gene delivery | - |
dc.type | Article | - |
dc.identifier.wosid | 000432873100005 | - |
dc.identifier.scopusid | 2-s2.0-85045482137 | - |
dc.type.rims | ART | - |
dc.citation.volume | 544 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 39 | - |
dc.citation.endingpage | 45 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF PHARMACEUTICS | - |
dc.identifier.doi | 10.1016/j.ijpharm.2018.04.015 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Choi, Joon Sig | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Electrostatic assembly | - |
dc.subject.keywordAuthor | Dendrimer | - |
dc.subject.keywordAuthor | Gene delivery | - |
dc.subject.keywordAuthor | Repebody | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | CANCER-THERAPY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TUMOR | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CARRIER | - |
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