Conessine Treatment Reduces Dexamethasone-Induced Muscle Atrophy by Regulating MuRF1 and Atrogin-1 Expression

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Conessine, a steroidal alkaloid, is a potent histamine H3 antagonist with antimalarial activity. We recently reported that conessine treatment interferes with H2O2-induced cell death by regulating autophagy. However, the cellular signaling pathways involved in conessine treatment are not fully understood. Here, we report that conessine reduces muscle atrophy by interfering with the expression of atrophy-related ubiquitin ligases MuRF-1 and atrogin-1. Promoter reporter assay revealed that conessine treatment inhibits FoxO3a-dependent transcription, NF-kappa B-dependent transcription, and p53-dependent transcription. We also showed by quantitative RT-PCR and western blot assays that conessine treatment reduced dexamethasone-induced expression of MuRF1 and atrogin-1. Finally, we demonstrated that conessine treatment reduced dexamethasone-induced muscle atrophy using differentiated C2C12 cells. These results collectively suggest that conessine is potentially useful in the treatment of muscle atrophy.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2018-04
Language
English
Article Type
Article
Keywords

SKELETAL-MUSCLE; UBIQUITIN LIGASES; TRANSCRIPTION FACTOR; PROTEIN-KINASE; TUMOR-CELLS; KAPPA-B; FOXO3A; APOPTOSIS; GROWTH; MAFBX/ATROGIN-1

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.28, no.4, pp.520 - 526

ISSN
1017-7825
DOI
10.4014/jmb.1711.11009
URI
http://hdl.handle.net/10203/242637
Appears in Collection
BS-Journal Papers(저널논문)
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