Architecture and substrate-recognition assembly of the Type IV coupling protein complex of L. pneumophila = 레지오넬라 뉴모필라 균의 제 4 유형 커플링 단백질 복합체의 구조 및 기질 인식 조립체

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Many intracellular bacteria, including Legionella pneumophila, rely on Type IV secretion system (T4SS) to translocate a repertoire of effector proteins into host cells for their survival and growth. T4CP is a hexameric ATPase that links translocating substrates to the transenvelope secretion conduit. Yet, how a large number of effector proteins are selectively recruited and processed by T4CPs remains enigmatic. DotL, the T4CP of L. pneumophila, contains an ATPase domain and a unique C-terminal extension whose function is unknown. Unlike T4CPs recruiting plasmid DNA for translocation, DotL appeared to function by forming a multi-protein complex together with four other proteins. Herein, we found that the C-terminal extension of DotL interacts with DotN, IcmS, IcmW and a newly identified subunit LvgA, and show that this pentameric assembly binds Legionella effector proteins. We determined the crystal structure of this assembly, and built an architecture of the T4CP holocomplex by combining a homology model of the N-terminal ATPase domain of DotL. The holocomplex is a hexamer of a bipartite structure composed of a membrane-proximal ATPase domain and a membrane-distal substrate-recognition assembly. The presented information represents the architecture and functional dissection of the multi-protein T4CP complexes in pathogenic bacteria, and provides important insights into their substrate recruitment and processing.
Advisors
Oh, Byung Haresearcher오병하researcher
Description
한국과학기술원 :생명과학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2017.8,[iv, 91 p. :]

Keywords

Legionella pneumophila▼aType IV secretion system▼aCoupling protein complex▼aEffector translocation▼aMolecular recognition; 레지오넬라 뉴모필라▼a제 4 유형 분비 체계▼a커플링 단백질 복합체▼a이펙터 전이▼a분자적 인식

URI
http://hdl.handle.net/10203/241857
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=718840&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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