DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박현규 | ko |
dc.contributor.author | 신성철 | ko |
dc.contributor.author | 김가희 | ko |
dc.contributor.author | 강병철 | ko |
dc.date.accessioned | 2017-12-20T01:43:47Z | - |
dc.date.available | 2017-12-20T01:43:47Z | - |
dc.date.issued | 2016-12-20 | - |
dc.identifier.uri | http://hdl.handle.net/10203/230001 | - |
dc.description.abstract | The present invention relates to a method for analyzing genes using SDL-PCR (separation of displaced ligation probe-based PCR), and more particularly to a method for analyzing genes using SDL-PCR, in which probes comprising a nucleotide sequence complementary to the gene of interest are ligated with each other by ligase, and another probe capable of hybridizing to the probes is hybridized and extended, thereby preparing a template probe, and the template probe for the gene of interest is amplified using universal primers. According to the SDL-PCR method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic DNA using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner. | - |
dc.title | Gene analysis method using SDL-PCR | - |
dc.title.alternative | SDL-PCR을 이용한 유전자 분석방법 | - |
dc.type | Patent | - |
dc.type.rims | PAT | - |
dc.contributor.localauthor | 박현규 | - |
dc.contributor.localauthor | 신성철 | - |
dc.contributor.nonIdAuthor | 김가희 | - |
dc.contributor.nonIdAuthor | 강병철 | - |
dc.contributor.assignee | KAIST | - |
dc.identifier.iprsType | 특허 | - |
dc.identifier.patentApplicationNumber | 14002662 | - |
dc.identifier.patentRegistrationNumber | 9523117 | - |
dc.date.application | 2012-01-30 | - |
dc.date.registration | 2016-12-20 | - |
dc.publisher.country | US | - |
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