DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ki Cheon | ko |
dc.contributor.author | Kumara, Madduma Hewage Susara Ruwan | ko |
dc.contributor.author | Kang, Kyoung Ah | ko |
dc.contributor.author | Piao, Mei Jing | ko |
dc.contributor.author | Oh, Min Chang | ko |
dc.contributor.author | Ryu, Yea Seong | ko |
dc.contributor.author | Jo, Jin Oh | ko |
dc.contributor.author | Mok, Young Sun | ko |
dc.contributor.author | Shin, Jennifer H. | ko |
dc.contributor.author | Park, Yeunsoo | ko |
dc.contributor.author | Kim, Seong Bong | ko |
dc.contributor.author | Yoo, Suk Jae | ko |
dc.contributor.author | Hyun, Jin Won | ko |
dc.date.accessioned | 2017-12-19T00:58:37Z | - |
dc.date.available | 2017-12-19T00:58:37Z | - |
dc.date.created | 2017-11-28 | - |
dc.date.created | 2017-11-28 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | MOLECULAR MEDICINE REPORTS, v.16, no.5, pp.6870 - 6875 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | http://hdl.handle.net/10203/228450 | - |
dc.description.abstract | Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA bases, including 8-oxogua-nine (8-oxoG). Our recent study reported that exposure of cells to non-thermal dielectric barrier discharge (DBD) plasma generates reactive oxygen species and damages DNA. The present study investigated the effect of non-thermal DBD plasma exposure on the formation of 8-oxoG in HaCaT human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 8-oxoG. In addition, mRNA and protein expression levels of 8-oxoguanine glycosylase 1 (OGG1), an 8-oxoG repair enzyme, were reduced in plasma-exposed cells. Furthermore, the expression level of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor that regulates OGG1 gene expression, was reduced following exposure to DBD plasma. Pretreatment of cells with an antioxidant, N-acetyl cysteine (NAC), prior to plasma exposure suppressed the formation of 8-oxoG and restored the expression levels of OGG1 and Nrf2. In addition, phosphorylation of protein kinase B (Akt), which regulates the activation of Nrf2, was reduced following plasma exposure. However, phosphorylation was restored by pretreatment with NAC. These findings suggested that non-thermal DBD plasma exposure generates 8-oxoG via inhibition of the Akt-Nrf2-OGG1 signaling pathway in HaCaT cells. | - |
dc.language | English | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | OGG1 GENE | - |
dc.subject | HUMAN HOMOLOG | - |
dc.subject | DNA | - |
dc.subject | CARCINOGENESIS | - |
dc.subject | GLYCOSYLASE | - |
dc.subject | PATHWAY | - |
dc.subject | CELLS | - |
dc.subject | 8-OXODEOXYGUANOSINE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | EXPRESSION | - |
dc.title | Exposure of keratinocytes to non-thermal dielectric barrier discharge plasma increases the level of 8-oxoguanine via inhibition of its repair enzyme | - |
dc.type | Article | - |
dc.identifier.wosid | 000414698900145 | - |
dc.identifier.scopusid | 2-s2.0-85030097870 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 6870 | - |
dc.citation.endingpage | 6875 | - |
dc.citation.publicationname | MOLECULAR MEDICINE REPORTS | - |
dc.identifier.doi | 10.3892/mmr.2017.7454 | - |
dc.contributor.localauthor | Shin, Jennifer H. | - |
dc.contributor.nonIdAuthor | Kim, Ki Cheon | - |
dc.contributor.nonIdAuthor | Kumara, Madduma Hewage Susara Ruwan | - |
dc.contributor.nonIdAuthor | Kang, Kyoung Ah | - |
dc.contributor.nonIdAuthor | Piao, Mei Jing | - |
dc.contributor.nonIdAuthor | Oh, Min Chang | - |
dc.contributor.nonIdAuthor | Ryu, Yea Seong | - |
dc.contributor.nonIdAuthor | Jo, Jin Oh | - |
dc.contributor.nonIdAuthor | Mok, Young Sun | - |
dc.contributor.nonIdAuthor | Park, Yeunsoo | - |
dc.contributor.nonIdAuthor | Kim, Seong Bong | - |
dc.contributor.nonIdAuthor | Yoo, Suk Jae | - |
dc.contributor.nonIdAuthor | Hyun, Jin Won | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | non-thermal dielectric barrier discharge plasma | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | 8-oxoguanine | - |
dc.subject.keywordAuthor | 8-oxoguanine glycosylase 1 | - |
dc.subject.keywordAuthor | nuclear factor erythroid 2-related factor 2 | - |
dc.subject.keywordPlus | OGG1 GENE | - |
dc.subject.keywordPlus | HUMAN HOMOLOG | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | CARCINOGENESIS | - |
dc.subject.keywordPlus | GLYCOSYLASE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | 8-OXODEOXYGUANOSINE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.