DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Jang, Hyowon | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2017-11-21T04:06:15Z | - |
dc.date.available | 2017-11-21T04:06:15Z | - |
dc.date.created | 2017-11-20 | - |
dc.date.created | 2017-11-20 | - |
dc.date.created | 2017-11-20 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | NANOSCALE, v.9, no.42, pp.16149 - 16153 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | http://hdl.handle.net/10203/227199 | - |
dc.description.abstract | We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on the target-triggered catalytic hairpin assembly (CHA) in conjunction with a G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In the absence of RNase H, the RNA/DNA duplex serving as a substrate for RNase H cannot initiate the execution of CHA that produces G-quadruplexes; so NMM shows a low fluorescence signal. In contrast, the presence of RNase H that degrades RNA in the RNA/DNA duplex releases DNA designed to function as the catalyst for CHA. This consequently promotes the efficient CHA and generates a large number of G-quadruplexes with a significantly enhanced fluorescence signal from NMM. Based on this label-free and enzyme-free signal amplification strategy, we successfully determined the RNase H activity with a detection limit of 0.037 U mL(-1) and screened potential RNase H inhibitors. Our results suggest that the developed system is a promising platform for a cost-effective, sensitive enzyme activity assay and inhibitor screening. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay | - |
dc.type | Article | - |
dc.identifier.wosid | 000414349100006 | - |
dc.identifier.scopusid | 2-s2.0-85032945516 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 42 | - |
dc.citation.beginningpage | 16149 | - |
dc.citation.endingpage | 16153 | - |
dc.citation.publicationname | NANOSCALE | - |
dc.identifier.doi | 10.1039/c7nr04060a | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RIBONUCLEASE-H | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | BIMOLECULAR BEACONS | - |
dc.subject.keywordPlus | AMPLIFIED DETECTION | - |
dc.subject.keywordPlus | KINETIC-ANALYSIS | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | INHIBITION | - |
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