DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jong-won | ko |
dc.contributor.author | Heu, Woosung | ko |
dc.contributor.author | Jeong, Sukyo | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2017-10-23T01:26:42Z | - |
dc.date.available | 2017-10-23T01:26:42Z | - |
dc.date.created | 2017-09-25 | - |
dc.date.created | 2017-09-25 | - |
dc.date.created | 2017-09-25 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.citation | ANALYTICA CHIMICA ACTA, v.988, pp.81 - 88 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/10203/226277 | - |
dc.description.abstract | Molecular detection of target molecules with high sensitivity and specificity is of great significance in bio and medical sciences. Here, we present genetically functionalized ferritin nanoparticles with a high-affinity protein binder, and their utility as a signal generator in a variety of immunoassays and imaging. As a high-affinity protein binder, human IgG-specific repebody, which is composed of LRR (Leucinerich repeat) modules, was used. The repebody was genetically fused to the N-terminal heavy-chain ferritin, and the resulting subunits were self-assembled to the repebody-ferritin nanoparticles composed of 24 subunits. The repebody-ferritin nanoparticles were shown to have a three-order of magnitude higher binding affinity toward human IgG than free repebody mainly owing to a decreased dissociation rate constant. The repebody-ferritin nanoparticles were conjugated with fluorescent dyes, and the resulting nanoparticles were used for western blotting, cell imaging, and flow cytometric analysis. The dye-labeled repebody-ferritin nanoparticles were shown to generate about 3-fold stronger fluorescent signals in immunoassays than monovalent repebody. The repebody-functionalized ferritin nanoparticles can be effectively used for sensitive and specific immunoassays and imaging in many areas. (C) 2017 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | CARBOHYDRATE ANTIGEN 125 | - |
dc.subject | SIGNAL AMPLIFICATION | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | ELECTROCHEMICAL IMMUNOASSAY | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | H-CHAIN | - |
dc.subject | BINDING | - |
dc.subject | APOFERRITIN | - |
dc.subject | CANCER | - |
dc.subject | EXPRESSION | - |
dc.title | Genetically functionalized ferritin nanoparticles with a high-affinity protein binder for immunoassay and imaging | - |
dc.type | Article | - |
dc.identifier.wosid | 000410338900010 | - |
dc.identifier.scopusid | 2-s2.0-85027417172 | - |
dc.type.rims | ART | - |
dc.citation.volume | 988 | - |
dc.citation.beginningpage | 81 | - |
dc.citation.endingpage | 88 | - |
dc.citation.publicationname | ANALYTICA CHIMICA ACTA | - |
dc.identifier.doi | 10.1016/j.aca.2017.07.060 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Repebody | - |
dc.subject.keywordAuthor | Ferritin | - |
dc.subject.keywordAuthor | Self-assembly | - |
dc.subject.keywordAuthor | Multivalency | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | Cell imaging | - |
dc.subject.keywordPlus | CARBOHYDRATE ANTIGEN 125 | - |
dc.subject.keywordPlus | SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | ELECTROCHEMICAL IMMUNOASSAY | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | H-CHAIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | APOFERRITIN | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | EXPRESSION | - |
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