Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization

Cited 54 time in webofscience Cited 0 time in scopus
  • Hit : 272
  • Download : 53
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms.
Publisher
CELL PRESS
Issue Date
2017-07
Language
English
Article Type
Article
Keywords

BACILLUS-SUBTILIS; PROKARYOTIC CONDENSIN; BACTERIAL CONDENSIN; MOLECULAR-BASIS; ATP HYDROLYSIS; B. SUBTILIS; DNA; COHESIN; HINGE; ORIGIN

Citation

MOLECULAR CELL, v.67, no.2, pp.334 - 347

ISSN
1097-2765
DOI
10.1016/j.molcel.2017.06.010
URI
http://hdl.handle.net/10203/225337
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
1707 Cell 논문 이한솔.pdf(5.89 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 54 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0