Identifying the cellular location of brain cytoplasmic 200 RNA using an RNA-recognizing antibody

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Brain cytoplasmic 200 RNA (BC200 RNA) is a neuron-specific non-coding RNA, implicated in the inhibition of local synaptodendritic protein synthesis, and is highly expressed in some cancer cells. Although BC200 RNA has been shown to inhibit translation in vitro, the cellular location of this inhibition is unknown. In this study, we used a BC200 RNA-recognizing antibody to identify the cellular locations of BC200 RNA in HeLa cervical carcinoma cells. We observed punctate signals in both the cytoplasm and nucleus, and further discovered that BC200 RNA co-localized with the p-body decapping enzyme, DCP1A, and the heterogeneous nuclear ribonucleoprotein E2 ( hnRNP E2). The latter is a known BC200 RNA-binding partner protein and a constituent of p-bodies. This suggests that BC200 RNA is localized to p-bodies via hnRNP E2.
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Issue Date
2017-06
Language
English
Article Type
Article
Keywords

DENDRITIC BC1 RNA; BINDING-PROTEIN 2; BC200 RNA; TRANSLATIONAL CONTROL; POLIOVIRUS RNA; BREAST-CANCER; RECOGNITION; EXPRESSION; INITIATION; MECHANISM

Citation

BMB REPORTS, v.50, no.6, pp.318 - 322

ISSN
1976-6696
DOI
10.5483/BMBRep.2017.50.6.217
URI
http://hdl.handle.net/10203/225158
Appears in Collection
CH-Journal Papers(저널논문)
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