With growing interests in using bacterial biopolymers in geotechnical practices, identifying mechanical properties of soft gel-like biopolymers is important in predicting their efficacy in soil modification and treatment. As one of the promising candidates, dextran was found to be produced by Leuconostoc mesenteroides. The model bacteria utilize sucrose as working material and synthesize both soluble and insoluble dextran which forms a complex and inhomogeneous polymer network. However, the traditional rheometer has a limitation to capture in situ properties of inherently porous and inhomogeneous biopolymers. Therefore, we used the particle tracking microrheology to characterize the material properties of the dextran polymer. TEM images revealed a range of pore size mostly less than 20 mu m, showing large pores > 2 mu m and small pores within the solid matrix whose sizes are less than 1 mu m. Microrheology data showed two distinct regimes in the bacterial dextran, purely viscous pore region of soluble dextran and viscoelastic region of the solid part of insoluble dextran matrix. Diffusive beads represented the soluble dextran dissolved in an aqueous phase, of which viscosity was three times higher than the growth medium viscosity. The local properties of the insoluble dextran were extracted from the results of the minimally moving beads embedded in the dextran matrix or trapped in small pores. At high frequency (omega> 0.2 Hz), the insoluble dextran showed the elastic behavior with the storage modulus of similar to 0.1 Pa. As frequency decreased, the insoluble dextran matrix exhibited the viscoelastic behavior with the decreasing storage modulus in the range of similar to 0.1-10(-3) Pa and the increasing loss modulus in the range of similar to 10(-4) -1 Pa. The obtained results provide a compilation of frequencydependent rheological or viscoelastic properties of soft gel-like porous biopolymers at the particular conditions where soil bacteria produce bacterial biopolymers in subsurface.