Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo

Cited 37 time in webofscience Cited 0 time in scopus
  • Hit : 494
  • Download : 0
Small interfering RNA (siRNA) is an attractive therapeutic candidate for sequence-specific gene silencing to treat incurable diseases using small molecule drugs. However, its efficient intracellular delivery has remained a challenge. Here, we have developed a highly biocompatible fluorescent carbon dot (CD), and demonstrate a functional siRNA delivery system that induces efficient gene knockdown in vitro and in vivo. We found that CD nanoparticles (NPs) enhance the cellular uptake of siRNA, via endocytosis in tumor cells, with low cytotoxicity and unexpected immune responses. Real-time study of fluorescence imaging in live cells shows that CD NPs favorably localize in cytoplasm and successfully release siRNA within 12 h. Moreover, we demonstrate that CD NP-mediated siRNA delivery significantly silences green fluorescence protein (GFP) expression and inhibits tumor growth in a breast cancer cell xenograft mouse model of tumor-specific therapy. We have developed a multifunctional siRNA delivery vehicle enabling simultaneous bioimaging and efficient downregulation of gene expression, that shows excellent potential for gene therapy.
Publisher
TSINGHUA UNIV PRESS
Issue Date
2017-02
Language
English
Article Type
Article
Keywords

LOW-MOLECULAR-WEIGHT; DOUBLE-STRANDED-RNA; MESOPOROUS SILICA NANOPARTICLES; MICROWAVE-ASSISTED PYROLYSIS; ENDOTHELIAL GROWTH-FACTOR; TARGETED SIRNA DELIVERY; HIGH QUANTUM YIELD; MAGNETIC NANOPARTICLES; ENHANCED-FLUORESCENCE; FUNCTIONAL DELIVERY

Citation

NANO RESEARCH, v.10, no.2, pp.503 - 519

ISSN
1998-0124
DOI
10.1007/s12274-016-1309-1
URI
http://hdl.handle.net/10203/222726
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 37 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0