Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo

Cited 36 time in webofscience Cited 0 time in scopus
  • Hit : 484
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorKim, Seongchanko
dc.contributor.authorChoi, Yuriko
dc.contributor.authorPark, Ginamko
dc.contributor.authorWon, Cheolheeko
dc.contributor.authorPark, Young-Joonko
dc.contributor.authorLee, Younghoonko
dc.contributor.authorKim, Byeong-Suko
dc.contributor.authorMin, Dal-Heeko
dc.date.accessioned2017-03-30T09:18:52Z-
dc.date.available2017-03-30T09:18:52Z-
dc.date.created2017-03-29-
dc.date.created2017-03-29-
dc.date.issued2017-02-
dc.identifier.citationNANO RESEARCH, v.10, no.2, pp.503 - 519-
dc.identifier.issn1998-0124-
dc.identifier.urihttp://hdl.handle.net/10203/222726-
dc.description.abstractSmall interfering RNA (siRNA) is an attractive therapeutic candidate for sequence-specific gene silencing to treat incurable diseases using small molecule drugs. However, its efficient intracellular delivery has remained a challenge. Here, we have developed a highly biocompatible fluorescent carbon dot (CD), and demonstrate a functional siRNA delivery system that induces efficient gene knockdown in vitro and in vivo. We found that CD nanoparticles (NPs) enhance the cellular uptake of siRNA, via endocytosis in tumor cells, with low cytotoxicity and unexpected immune responses. Real-time study of fluorescence imaging in live cells shows that CD NPs favorably localize in cytoplasm and successfully release siRNA within 12 h. Moreover, we demonstrate that CD NP-mediated siRNA delivery significantly silences green fluorescence protein (GFP) expression and inhibits tumor growth in a breast cancer cell xenograft mouse model of tumor-specific therapy. We have developed a multifunctional siRNA delivery vehicle enabling simultaneous bioimaging and efficient downregulation of gene expression, that shows excellent potential for gene therapy.-
dc.languageEnglish-
dc.publisherTSINGHUA UNIV PRESS-
dc.subjectLOW-MOLECULAR-WEIGHT-
dc.subjectDOUBLE-STRANDED-RNA-
dc.subjectMESOPOROUS SILICA NANOPARTICLES-
dc.subjectMICROWAVE-ASSISTED PYROLYSIS-
dc.subjectENDOTHELIAL GROWTH-FACTOR-
dc.subjectTARGETED SIRNA DELIVERY-
dc.subjectHIGH QUANTUM YIELD-
dc.subjectMAGNETIC NANOPARTICLES-
dc.subjectENHANCED-FLUORESCENCE-
dc.subjectFUNCTIONAL DELIVERY-
dc.titleHighly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo-
dc.typeArticle-
dc.identifier.wosid000394322300013-
dc.identifier.scopusid2-s2.0-84994448306-
dc.type.rimsART-
dc.citation.volume10-
dc.citation.issue2-
dc.citation.beginningpage503-
dc.citation.endingpage519-
dc.citation.publicationnameNANO RESEARCH-
dc.identifier.doi10.1007/s12274-016-1309-1-
dc.contributor.localauthorLee, Younghoon-
dc.contributor.nonIdAuthorKim, Seongchan-
dc.contributor.nonIdAuthorChoi, Yuri-
dc.contributor.nonIdAuthorPark, Ginam-
dc.contributor.nonIdAuthorWon, Cheolhee-
dc.contributor.nonIdAuthorPark, Young-Joon-
dc.contributor.nonIdAuthorKim, Byeong-Su-
dc.contributor.nonIdAuthorMin, Dal-Hee-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorbioimaging-
dc.subject.keywordAuthorcarbon dot-
dc.subject.keywordAuthorgene delivery-
dc.subject.keywordAuthorRNA interference-
dc.subject.keywordAuthortargeted cancer therapy-
dc.subject.keywordPlusLOW-MOLECULAR-WEIGHT-
dc.subject.keywordPlusDOUBLE-STRANDED-RNA-
dc.subject.keywordPlusMESOPOROUS SILICA NANOPARTICLES-
dc.subject.keywordPlusMICROWAVE-ASSISTED PYROLYSIS-
dc.subject.keywordPlusENDOTHELIAL GROWTH-FACTOR-
dc.subject.keywordPlusTARGETED SIRNA DELIVERY-
dc.subject.keywordPlusHIGH QUANTUM YIELD-
dc.subject.keywordPlusMAGNETIC NANOPARTICLES-
dc.subject.keywordPlusENHANCED-FLUORESCENCE-
dc.subject.keywordPlusFUNCTIONAL DELIVERY-
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 36 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0