Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina = 전사인자 과발현을 통한 나노클로롭시스 살리나의 바이오매스와 지질 생산 향상 연구

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TFs with basic leucine zipper (bZIP) domain have been identified as stress regulators and associated with regulating lipid metabolism. Normal condition and stress conditions were employed to confirm effects in NsbZIP1 overexpression strains. In our study, we overexpressed NsbZIP1 in N. salina CCMP1776 and confirmed by genomic PCR and western blotting. Transformants showed conspicuous increase under stress conditions. NsbZIP1 expression enhanced DCW and lipid content under stress conditions. NsbZIP1 transformants not only were able to maintain high electron transport rate, but also showed enhanced NPQ and Fv/Fm values. This results indicated that NsbZIP1 expression may be involved in the regulation and improvement of stress resistance as stress regulators. Also, NsbZIP1 activity accelerates TAG biosynthesis pathway by upregulating lipid-metabolizing genes specifically. We confirmed that NsbZIP1 activity play a widespread roles in mediating stress signaling as stress regulators and regulating growth and expression of gene related to lipid metabolism.
Advisors
Chang, Yong Keunresearcher장용근researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2016.8 ,[v, 54 p. :]

Keywords

Nannochloropsis salina; Basic region/leucine zipper transcription factor (bZIP TF); Growth rate; FAME productivity; Stress regulator; 나노클로롭시스 살리나; 비집 전사인자; 성장률; 지질 생산성; 스트레스 조절인자

URI
http://hdl.handle.net/10203/221521
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=663371&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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