DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Ji Young | ko |
dc.contributor.author | Yang, Keum-Jin | ko |
dc.contributor.author | Park, Shi-Nae | ko |
dc.contributor.author | Kim, Dong-Kee | ko |
dc.contributor.author | Kim, Jong-Duk | ko |
dc.date.accessioned | 2017-01-05T07:57:39Z | - |
dc.date.available | 2022-06-02T21:01:00Z | - |
dc.date.created | 2016-12-13 | - |
dc.date.created | 2016-12-13 | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF NANOMEDICINE, v.11, pp.6123 - 6134 | - |
dc.identifier.issn | 1178-2013 | - |
dc.identifier.uri | http://hdl.handle.net/10203/216108 | - |
dc.description.abstract | "Dexamethasone (Dex)-loaded PHEA-g-C18-Arg8 (PCA) nanoparticles (PCA/Dex) were developed for the delivery of genes to determine the synergistic effect of Dex on gene expression. The cationic PCA nanoparticles were self-assembled to create cationic micelles containing an octadecylamine (C18) core with Dex and an arginine 8 (Arg8) peptide shell for electrostatic complexation with nucleic acids (connexin 26 [Cx26] siRNA, green fluorescent protein [GFP] DNA or brain-derived neurotrophic factor [BDNF] pDNA). The PCA/Dex nanoparticles conjugated with Arg8, a cell-penetrating peptide that enhances permeability through a round window membrane in the inner ear for gene delivery, exhibited high uptake efficiency in HEI-OC1 cells. This potential carrier co-delivering Dex and the gene into inner ear cells has a diameter of 120-140 nm and a zeta potential of 20-25 mV. Different types of genes were complexed with the Dex-loaded PCA nanoparticle (PCA/Dex/gene) for gene expression to induce additional anti-inflammatory effects. PCA/Dex showed mildly increased expression of GFP and lower mRNA expression of inflammatory cytokines (IL1b, IL12, and INFr) than did Dex-free PCA nanoparticles and Lipofectamine (R) reagent in HEI-OC1 cells. In addition, after loading Cx26 siRNA onto the surface of PCA/Dex, Cx26 gene expression was downregulated according to real-time polymerase chain reaction for 24 h, compared with that using Lipofectamine reagent. After loading BDNF DNA into PCA/Dex, increased expression of BDNF was observed for 30 h, and its signaling pathway resulted in an increase in phosphorylation of Akt, observed by Western blotting. Thus, Dex within PCA/Dex/gene nanoparticles created an anti-inflammatory effect and enhanced gene expression. | - |
dc.language | English | - |
dc.publisher | DOVE MEDICAL PRESS LTD | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | TARGETED DELIVERY | - |
dc.subject | MOUSE | - |
dc.subject | CELLS | - |
dc.subject | TRANSLOCATION | - |
dc.subject | POLYMERSOMES | - |
dc.subject | MEMBRANE | - |
dc.subject | MICELLES | - |
dc.subject | BARRIER | - |
dc.subject | CARRIER | - |
dc.title | The effect of dexamethasone/cell-penetrating peptide nanoparticles on gene delivery for inner ear therapy | - |
dc.type | Article | - |
dc.identifier.wosid | 000387820700001 | - |
dc.identifier.scopusid | 2-s2.0-84996489213 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.beginningpage | 6123 | - |
dc.citation.endingpage | 6134 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.identifier.doi | 10.2147/IJN.S114241 | - |
dc.embargo.terms | 2017-04-02 | - |
dc.contributor.localauthor | Kim, Jong-Duk | - |
dc.contributor.nonIdAuthor | Yang, Keum-Jin | - |
dc.contributor.nonIdAuthor | Park, Shi-Nae | - |
dc.contributor.nonIdAuthor | Kim, Dong-Kee | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell-penetrating peptide | - |
dc.subject.keywordAuthor | nanoparticle | - |
dc.subject.keywordAuthor | dexamethasone | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | brain-derived neurotrophic factor | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | TARGETED DELIVERY | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | POLYMERSOMES | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | MICELLES | - |
dc.subject.keywordPlus | BARRIER | - |
dc.subject.keywordPlus | CARRIER | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.