Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins

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dc.contributor.authorHan, MJko
dc.contributor.authorLee, JWko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2010-12-24T05:44:16Z-
dc.date.available2010-12-24T05:44:16Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2005-11-
dc.identifier.citationJOURNAL OF PROTEOME RESEARCH, v.4, pp.2429 - 2434-
dc.identifier.issn1535-3893-
dc.identifier.urihttp://hdl.handle.net/10203/21227-
dc.description.abstractProteolytic degradation is one of the critical problems in two-dimensional electrophoresis (2-DE). Here we report that small heat shock proteins (sHsps), including IbpA(Ec) and IbpB(Ec) from Escherichia coli and Hsp26(Sc) from Saccharomyces cerevisiae, are able to protect proteins in vitro from proteolytic degradation. Addition of sHsps during 2-DE of human serum or whole cell extracts of E. coli, Mannheimia succinciproducens, Arabidopsis thaliana, and human kidney cells allowed detection of up to 50% more protein spots than those obtainable with currently available protease inhibitors. Therefore, the use of sHsps during 2-DE significantly improves proteome profiling by generally enabling the detection of many more protein spots that could not be seen previously.-
dc.description.sponsorshipWe thank Cancer Metastasis Research Center at Yonsei University and Prof. W. I. Chung for providing us with human serum samples and A. thaliana leaves, respectively. This work was supported by a Korean Systems Biology Research Grant (M10309020000-03B5002-00000) from the Korean Ministry of Science and Technology. Additional support was provided by the LG Chem Chair Professorship, the Brain Korea 21 project, and the Center for Ultramicrochemical Process Systems.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherAMER CHEMICAL SOC-
dc.subject2-DIMENSIONAL GEL-ELECTROPHORESIS-
dc.subjectESCHERICHIA-COLI-
dc.subjectPLANT-TISSUES-
dc.subjectEXPRESSION-
dc.subjectIBPB-
dc.titleEnhanced proteome profiling by inhibiting proteolysis with small heat shock proteins-
dc.typeArticle-
dc.identifier.wosid000234007200064-
dc.identifier.scopusid2-s2.0-29144523875-
dc.type.rimsART-
dc.citation.volume4-
dc.citation.beginningpage2429-
dc.citation.endingpage2434-
dc.citation.publicationnameJOURNAL OF PROTEOME RESEARCH-
dc.identifier.doi10.1021/pr050259m-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorHan, MJ-
dc.contributor.nonIdAuthorLee, JW-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorsmall heat shock proteins-
dc.subject.keywordAuthortwo-dimensional gel electrophoresis-
dc.subject.keywordAuthorproteome profiling-
dc.subject.keywordAuthorprotease inhibitor-
dc.subject.keywordPlus2-DIMENSIONAL GEL-ELECTROPHORESIS-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusPLANT-TISSUES-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusIBPB-
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