DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dae-Geun | ko |
dc.contributor.author | Setiaputra, Dheva | ko |
dc.contributor.author | Jung, Tae Yang | ko |
dc.contributor.author | Chung, Jae Hee | ko |
dc.contributor.author | Leitner, Alexander | ko |
dc.contributor.author | Yoon, Jung Min | ko |
dc.contributor.author | Aebersold, Ruedi | ko |
dc.contributor.author | Hebert, Hans | ko |
dc.contributor.author | Yip, Calvin K. | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.date.accessioned | 2016-07-07T04:55:58Z | - |
dc.date.available | 2016-07-07T04:55:58Z | - |
dc.date.created | 2016-06-21 | - |
dc.date.created | 2016-06-21 | - |
dc.date.created | 2016-06-21 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.6 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209731 | - |
dc.description.abstract | Chromatin Assembly Complex 1 (CAF-1) is a major histone chaperone involved in deposition of histone H3 and H4 into nucleosome. CAF-1 is composed of three subunits; p150, p60 and p48 for human and Cac1, Cac2 and Cac3 for yeast. Despite of its central role in chromatin formation, structural features of the full CAF-1 in complex with histones and other chaperones have not been well characterized. Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that Cac1, the largest subunit of yCAF-1, might serve as a major histone binding platform linking Cac2 and Cac3. In addition, EM analysis showed that yCAF-1 adopts a bilobal shape and Cac1 connecting Cac2 and Cac3 to generate a platform for binding histones. This study provides the first structural glimpse of the full CAF-1 complex and a structural framework to understand histone chaperoning processes | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Molecular Architecture of Yeast Chromatin Assembly Factor 1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000376499900001 | - |
dc.identifier.scopusid | 2-s2.0-84971317627 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep26702 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Setiaputra, Dheva | - |
dc.contributor.nonIdAuthor | Leitner, Alexander | - |
dc.contributor.nonIdAuthor | Aebersold, Ruedi | - |
dc.contributor.nonIdAuthor | Hebert, Hans | - |
dc.contributor.nonIdAuthor | Yip, Calvin K. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NEWLY SYNTHESIZED HISTONES | - |
dc.subject.keywordPlus | DNA-REPLICATION | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | METHYLTRANSFERASE ACTIVITY | - |
dc.subject.keywordPlus | H4 | - |
dc.subject.keywordPlus | ASF1 | - |
dc.subject.keywordPlus | ACETYLATION | - |
dc.subject.keywordPlus | CAF-1 | - |
dc.subject.keywordPlus | H3 | - |
dc.subject.keywordPlus | DEPOSITION | - |
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