Multiplexed Detection of Epigenetic Markers Using Quantum Dot (QD)-Encoded Hydrogel Microparticles

Cited 14 time in webofscience Cited 0 time in scopus
  • Hit : 388
  • Download : 0
Epigenetic alterations in gene expression are influenced by experiences and environment, resulting in significant variation of epigenetic markers from individual to individual. Therefore, it is imperative to measure various epigenetic markers simultaneously from samples of individual subjects to accurately analyze the epigenetic markers in biological samples. Moreover, the individualized genome-wide analysis has become a critical technology for recent trends in clinical applications such as early diagnosis and personalized medicine screening of numerous diseases. The array-based detection of modified histones, conventionally used for multiplexed analysis of epigenetic changes, requires pooling of samples from many subjects to analyze population wise differences in the expression of histone markers and does not permit individualized analysis. Here, we report multiplexed detection of genome-wide changes in various histone modifications at a single-residue resolution using quantum dot (QP)-encoded polyethylene glycol diacrylate (PEGDA) hydrogel microparticles. To demonstrate the potential of our methodology, we present the simultaneous detection of (I) acetylation of lysine 9 of histone 3 (Ac-H3K9), dimethylation of H3K9 (2Me-H3K9), and (3) trimethylation of H3K9 (3Me-H3K9) from three distinct regions in the brain [nucleus accumbens (NAc), dorsal striatum (DSt), and cerebellum (Cbl)] of cocaine-exposed mice. Our hydroger-based epigenetic assay enabled relative quantification of the three histone variants from only 10 mu L of each brain lysate (protein content = similar to 1 mu g/mu L) per mouse. We verified that the exposure to cocaine induced a significant increase of acetylation while a notable decrease in methylation in NAc
Publisher
AMER CHEMICAL SOC
Issue Date
2016-04
Language
English
Article Type
Article
Keywords

COCAINE-INDUCED PLASTICITY; NUCLEUS-ACCUMBENS; HISTONE MODIFICATION; PROTEIN-DETECTION; DRUG-ADDICTION; PARTICLES; CHROMATIN; QUANTIFICATION; ACETYLATION; METHYLATION

Citation

ANALYTICAL CHEMISTRY, v.88, no.8, pp.4259 - 4268

ISSN
0003-2700
DOI
10.1021/acs.analchem.5b04190
URI
http://hdl.handle.net/10203/209535
Appears in Collection
EE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 14 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0