DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Dae-Woong | ko |
dc.contributor.author | Kim, KyuHan | ko |
dc.contributor.author | Choi, Myung-Chul | ko |
dc.contributor.author | Choi, Siyoung Q. | ko |
dc.date.accessioned | 2016-06-07T09:16:41Z | - |
dc.date.available | 2016-06-07T09:16:41Z | - |
dc.date.created | 2016-02-22 | - |
dc.date.created | 2016-02-22 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.104 | - |
dc.identifier.issn | 1940-087X | - |
dc.identifier.uri | http://hdl.handle.net/10203/207860 | - |
dc.description.abstract | We introduce a new method to measure the lateral diffusivity of a surfactant monolayer at the fluid-fluid interface, called fluorescence recovery after merging (FRAM). FRAM adopts the same principles as the fluorescence recovery after photobleaching (FRAP) technique, especially for measuring fluorescence recovery after bleaching a specific area, but FRAM uses a drop coalescence instead of photobleaching dye molecules to induce a chemical potential gradient of dye molecules. Our technique has several advantages over FRAP: it only requires a fluorescence microscope rather than a confocal microscope equipped with high power lasers; it is essentially free from the selection of fluorescence dyes; and it has far more freedom to define the measured diffusion area. Furthermore, FRAM potentially provides a route for studying the mixing or inter-diffusion of two different surfactants, when the monolayers at a surface of droplet and at a flat air/water interface are prepared with different species, independently. | - |
dc.language | English | - |
dc.publisher | JOURNAL OF VISUALIZED EXPERIMENTS | - |
dc.subject | TRANSLATIONAL DIFFUSION | - |
dc.subject | LATERAL DIFFUSION | - |
dc.subject | MEMBRANE | - |
dc.subject | MICRORHEOLOGY | - |
dc.subject | CHOLESTEROL | - |
dc.subject | SURFACTANT | - |
dc.subject | FLUIDITY | - |
dc.subject | LIPIDS | - |
dc.title | Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer | - |
dc.type | Article | - |
dc.identifier.wosid | 000368572800053 | - |
dc.identifier.scopusid | 2-s2.0-84946429575 | - |
dc.type.rims | ART | - |
dc.citation.issue | 104 | - |
dc.citation.publicationname | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | - |
dc.identifier.doi | 10.3791/53376 | - |
dc.contributor.localauthor | Choi, Myung-Chul | - |
dc.contributor.localauthor | Choi, Siyoung Q. | - |
dc.contributor.nonIdAuthor | Jeong, Dae-Woong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bioengineering | - |
dc.subject.keywordAuthor | Issue 104 | - |
dc.subject.keywordAuthor | FRAM | - |
dc.subject.keywordAuthor | diffusion | - |
dc.subject.keywordAuthor | drop coalescence | - |
dc.subject.keywordAuthor | phospholipid monolayer | - |
dc.subject.keywordAuthor | FRAP | - |
dc.subject.keywordAuthor | inter-diffusion | - |
dc.subject.keywordAuthor | fluid-fluid interfaces | - |
dc.subject.keywordPlus | TRANSLATIONAL DIFFUSION | - |
dc.subject.keywordPlus | LATERAL DIFFUSION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | MICRORHEOLOGY | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | SURFACTANT | - |
dc.subject.keywordPlus | FLUIDITY | - |
dc.subject.keywordPlus | LIPIDS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.