Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

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dc.contributor.authorJeong, Dae-Woongko
dc.contributor.authorKim, KyuHanko
dc.contributor.authorChoi, Myung-Chulko
dc.contributor.authorChoi, Siyoung Q.ko
dc.date.accessioned2016-06-07T09:16:41Z-
dc.date.available2016-06-07T09:16:41Z-
dc.date.created2016-02-22-
dc.date.created2016-02-22-
dc.date.issued2015-10-
dc.identifier.citationJOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.104-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10203/207860-
dc.description.abstractWe introduce a new method to measure the lateral diffusivity of a surfactant monolayer at the fluid-fluid interface, called fluorescence recovery after merging (FRAM). FRAM adopts the same principles as the fluorescence recovery after photobleaching (FRAP) technique, especially for measuring fluorescence recovery after bleaching a specific area, but FRAM uses a drop coalescence instead of photobleaching dye molecules to induce a chemical potential gradient of dye molecules. Our technique has several advantages over FRAP: it only requires a fluorescence microscope rather than a confocal microscope equipped with high power lasers; it is essentially free from the selection of fluorescence dyes; and it has far more freedom to define the measured diffusion area. Furthermore, FRAM potentially provides a route for studying the mixing or inter-diffusion of two different surfactants, when the monolayers at a surface of droplet and at a flat air/water interface are prepared with different species, independently.-
dc.languageEnglish-
dc.publisherJOURNAL OF VISUALIZED EXPERIMENTS-
dc.subjectTRANSLATIONAL DIFFUSION-
dc.subjectLATERAL DIFFUSION-
dc.subjectMEMBRANE-
dc.subjectMICRORHEOLOGY-
dc.subjectCHOLESTEROL-
dc.subjectSURFACTANT-
dc.subjectFLUIDITY-
dc.subjectLIPIDS-
dc.titleFluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer-
dc.typeArticle-
dc.identifier.wosid000368572800053-
dc.identifier.scopusid2-s2.0-84946429575-
dc.type.rimsART-
dc.citation.issue104-
dc.citation.publicationnameJOVE-JOURNAL OF VISUALIZED EXPERIMENTS-
dc.identifier.doi10.3791/53376-
dc.contributor.localauthorChoi, Myung-Chul-
dc.contributor.localauthorChoi, Siyoung Q.-
dc.contributor.nonIdAuthorJeong, Dae-Woong-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorBioengineering-
dc.subject.keywordAuthorIssue 104-
dc.subject.keywordAuthorFRAM-
dc.subject.keywordAuthordiffusion-
dc.subject.keywordAuthordrop coalescence-
dc.subject.keywordAuthorphospholipid monolayer-
dc.subject.keywordAuthorFRAP-
dc.subject.keywordAuthorinter-diffusion-
dc.subject.keywordAuthorfluid-fluid interfaces-
dc.subject.keywordPlusTRANSLATIONAL DIFFUSION-
dc.subject.keywordPlusLATERAL DIFFUSION-
dc.subject.keywordPlusMEMBRANE-
dc.subject.keywordPlusMICRORHEOLOGY-
dc.subject.keywordPlusCHOLESTEROL-
dc.subject.keywordPlusSURFACTANT-
dc.subject.keywordPlusFLUIDITY-
dc.subject.keywordPlusLIPIDS-
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BiS-Journal Papers(저널논문)CBE-Journal Papers(저널논문)
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